Team:WashU/Week8

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We repeated the digests and PCRs from yesterday in order to ascertain what went wrong. The PCRs yielded primer dimers and were thus unsuccessful, so we plan on troubleshooting our procedure to determine why our reactions are failing.
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[PICTURE OF GEL]
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Revision as of 20:09, 17 July 2012



Monday, July 16

We ran multiple digests today, following the standard biobrick assembly protocol.

Digestions
Z and C: digest with P and E
Ligation 2: digest with X and P
Ligation 2: digest with X
Z and C: digest with E
ZCD: digest with X and P
UGTCs2: digest with X and P
2: digest with P
2: digest with B

The gel of these digests can be shown below. [INSERT GEL PICTURE HERE]

In addition, we ran several PCRs today. Following the NEB protocol, we ran four PCRs to amplify the CCD plasmid (PUT PROPER NAME HERE) and also tried four colony PCRs, using colonies 2, 4, 13 and ______.

To enable amplification of our plasmid PSL2131, PsbA2, and CS42S, we designed primers to multiply the aforementioned DNA pieces.
Tuesday, July 17

We repeated the digests and PCRs from yesterday in order to ascertain what went wrong. The PCRs yielded primer dimers and were thus unsuccessful, so we plan on troubleshooting our procedure to determine why our reactions are failing. [PICTURE OF GEL]


Wednesday, July 18


Thursday, July 19


Friday, July 20


Back to Weekly Log

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