Team:Nanjing-China

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|Nanjing_China is an iGEM team formed by undergraduate students of Nanjing university, most of whom are in the school of life science. We will be a team focusing on micro RNA and vegetable in this summer.
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''This project is about miRNA, vegetable and disease. It is observed that there are plant-origin microRNAs flowing in our blood.And these RNAs is obtained through our daily diet. We are trying to make use of this way to make micro-RNA producing vegetable, hoping to deal with tough disease by regulating gene expression.   
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|align="center"|[[Team:Nanjing-China | Team Nanjing-China]]
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          <h2>Abstract</h2>
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          <p>We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.</p>
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          <p>We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.</p>
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          <p>In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.</p>
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Revision as of 13:21, 5 September 2012

<!DOCTYPE html> Team:Nanjing-China

NanjingChina

Abstract

We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.

We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.

In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.

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