Team:Exeter

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Please visit our <a href="https://2012.igem.org/Team:Exeter">revised page</a> for all ExiGEM news.
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      <p><a href="http://www.wellcome.ac.uk/index.htm"><img src="https://static.igem.org/mediawiki/igem.org/0/03/Exe2012Wellcometrustlogo.png" width="200px" alt="Find out more about the Wellcome
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      Trust" title="Find out more about the Wellcome Trust" /></a>
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      </p>
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      <p>&nbsp;</p>
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      <p><a href="http://www.exeter.ac.uk/alumnisupporters/supporting/how/makeagift/annualfund/"><img src="https://static.igem.org/mediawiki/igem.org/f/fc/Exe2012Annualfundlogo.png" align="middle"
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      width="200px" alt="Find out how to donate to the Exeter University Annual Fund" title="Find out how to donate to the Exeter University Annual Fund" /></a>
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      </p>
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      <p>&nbsp;</p>
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      <p><font face="DokChampa" color="#1d1d1b" size="2"><b>With Kind Contribution From:</b></font>
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      </p>
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      <font face="DokChampa" color="#1d1d1b" size="2"><li><a href="http://biosciences.exeter.ac.uk/staff/index.php?web_id=murray_grant" style="color:#1d1d1b">Professor Murray Grant
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      </a></li><li><a href="http://biosciences.exeter.ac.uk/staff/index.php?web_id=ken_haynes" style="color:#1d1d1b">Professor Ken Haynes</a></li><li><a href=
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      "http://biosciences.exeter.ac.uk/staff/index.php?web_id=Rob_Beardmore" style="color:#1d1d1b">Professor Robert Beardmore</a></li>
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      <p>&nbsp;</p>
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      <img src="http://www4.clustrmaps.com/counter/index2.php?url=https://2012.igem.org/Team:Exeter" align="middle" style="border:0px;" alt="Locations of visitors to this
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      page" title="See who has been looking at ExiGEM's e-candi!" id="clustrMapsImg" />
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      <font face="DokChampa" color="#1d1d1b" size="+2"><p><b>Latest News:</b></p></font>
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      <!--Abstract-->
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      <font face="DokChampa" color="#1d1d1b" size="+2"><b>Project Abstract:</b>
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        <p>Our aim is to design a system for the synthesis of bespoke polysaccharides in <em>Escherichia  coli</em>. We intend to show that a future where designer polysaccharides
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        can be created on demand is not only  feasible, but in fact highly achievable.</p>
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        <p>The ability to tailor-make polysaccharides would lead to revolutionary technological developments in many fields, such as medicine (rapid vaccine production) and the
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        industrial sector (production of novel materials) to name but two.</p>
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        <p>We are using several approaches to demonstrate how this is possible. Firstly we want to synthesise useful existing polysaccharides (such as hyaluronan) in the unfamiliar
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        setting of <em>E. coli</em>. Secondly, to achieve the synthetic  polysaccharide, we will implement the addition of glycosyltransferases  from different <em>E. Coli</em> to
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        our own sample strain, both individually for characterisation and in an operon, allowing polysaccharide production via the well documented Wzy-dependent system.
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        As well as implementing a full operon for each polysaccharide, we plan to construct an engineered plasmid. This will  allow controlled expression of different enzymes,
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        induced through different promoters to enable choice without repeated genetic transformations..</p>
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        <p>The complementary dry lab work provides a basis for that of the wet lab and the principle of in vivo synthesis of novel polysaccharides. The generation of a database of
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        enzymes with a user-friendly interface will aim to assist polysaccharide design, leading to a future where any polysaccharide is possible. We are also investigating kinetic
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        models to flag up conflicts in enzyme activities to optimise polysaccharide construction pathways, and we plan to use TinkerCell to explore possible methods of controlling
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        multiple enzyme expression and enzyme expression levels.</p>
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Revision as of 12:04, 19 July 2012

Please visit our revised page for all ExiGEM news.

Retrieved from "http://2012.igem.org/Team:Exeter"