Team:Cambridge/Week 3/Lab book

From 2012.igem.org

(Difference between revisions)
Line 48: Line 48:
Making ''bacillus'' competent
Making ''bacillus'' competent
 +
 +
----
 +
 +
===Wednesday===
 +
 +
Making ''bacillus'' competent
 +
 +
----
 +
 +
===Thursday===
 +
 +
Test transformation of frozen ''bacillus'' stocks
 +
 +
----
 +
 +
===Friday===
 +
 +
Transformation of ''bacillus'' with lux genes from 2010
----
----

Revision as of 13:50, 13 July 2012

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions Sponsors


Diary Lab book


Contents

Monday

Making bacillus competent


Bacillus salts *10 made up:

  • (NH4)2SO4: 20g
  • KH2PO4 (anhydrous): 60g
  • Anhydrous K2HPO4 189.7g (N.B. Different from the one below!)
  • KH2PO4 60g
  • Tri-sodium citrate 10g
  • MgSO4•7H2O 2g
  • Added distilled water to make 1000mL
  • Autoclaved

Medium A base *10 made up:

  • Yeast extract 10g
  • Casamino acids 2g
  • Added distilled water to make 900mL
  • Autoclaved

bacillus strain 168 streaked out and grown on plate overnight.

Tuesday

Making bacillus competent


Wednesday

Making bacillus competent


Thursday

Test transformation of frozen bacillus stocks


Friday

Transformation of bacillus with lux genes from 2010