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Team:Cambridge/Week 3/Lab book
From 2012.igem.org
(Difference between revisions)
| Line 29: | Line 29: | ||
*(NH4)2SO4: 20g | *(NH4)2SO4: 20g | ||
* KH2PO4 (anhydrous): 60g | * KH2PO4 (anhydrous): 60g | ||
| + | * Anhydrous K2HPO4 189.7g ''(N.B. Different from the one below!)'' | ||
| + | * KH2PO4 60g | ||
| + | * Tri-sodium citrate 10g | ||
| + | * MgSO4•7H2O 2g | ||
| + | * SDW to 1000mL | ||
| + | |||
| + | ''Medium A base'' *10 made up: | ||
Revision as of 13:38, 13 July 2012
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Monday
Making bacillus competent
Bacillus salts *10 made up:
- (NH4)2SO4: 20g
- KH2PO4 (anhydrous): 60g
- Anhydrous K2HPO4 189.7g (N.B. Different from the one below!)
- KH2PO4 60g
- Tri-sodium citrate 10g
- MgSO4•7H2O 2g
- SDW to 1000mL
Medium A base *10 made up:
