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Team:Nevada/Protocols
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{{Template:Team:Nevada:Final_Header}} | {{Template:Team:Nevada:Final_Header}} | ||
<br><br> | <br><br> | ||
| - | <p>[[Team:Nevada/Notebook|Back to Notebook]] . [[Team:Nevada/Recipes|View Recipes]]<br/><br/ | + | <p>[[Team:Nevada/Notebook|Back to Notebook]] . [[Team:Nevada/Recipes|View Recipes]]<br/><br/> |
==Phusion PCR== | ==Phusion PCR== | ||
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* 55°C, 60°C, 65°C for 15 seconds | * 55°C, 60°C, 65°C for 15 seconds | ||
* 72°C for 15 seconds | * 72°C for 15 seconds | ||
| - | Final Extension: 72°C for 5 minutes<br/ | + | Final Extension: 72°C for 5 minutes<br/> |
==Qiagen Miniprep kit== | ==Qiagen Miniprep kit== | ||
www.qiagen.com/hb/qiaprepminiprep<br/> | www.qiagen.com/hb/qiaprepminiprep<br/> | ||
Revision as of 19:17, 12 July 2012
Back to Notebook . View Recipes
Phusion PCR
Thermocycling conditions:
Initial Denaturation: 98°C for 30 seconds
25-35 cycles:
- 98°C for 10 seconds
- 55°C, 60°C, 65°C for 15 seconds
- 72°C for 15 seconds
Final Extension: 72°C for 5 minutes
