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Team:Nevada/Protocols
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{{Template:Team:Nevada:Final_Header}} | {{Template:Team:Nevada:Final_Header}} | ||
<br><br> | <br><br> | ||
| - | <p>[[Team:Nevada/Notebook|Back to Notebook]] . [[Team:Nevada/Recipes|View Recipes]]<br/><br/> | + | <p>[[Team:Nevada/Notebook|Back to Notebook]] . [[Team:Nevada/Recipes|View Recipes]]<br/><br/><p> |
| - | <p> | + | |
| - | + | ==Phusion PCR== | |
Thermocycling conditions:<br/> | Thermocycling conditions:<br/> | ||
Initial Denaturation: 98°C for 30 seconds <br/> | Initial Denaturation: 98°C for 30 seconds <br/> | ||
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* 55°C, 60°C, 65°C for 15 seconds | * 55°C, 60°C, 65°C for 15 seconds | ||
* 72°C for 15 seconds | * 72°C for 15 seconds | ||
| - | Final Extension: 72°C for 5 minutes<br/> | + | Final Extension: 72°C for 5 minutes<br/> <p> |
| - | + | ==Qiagen Miniprep kit== | |
| - | + | ||
www.qiagen.com/hb/qiaprepminiprep<br/> | www.qiagen.com/hb/qiaprepminiprep<br/> | ||
Revision as of 19:16, 12 July 2012
Back to Notebook . View Recipes
<p>
Phusion PCR
Thermocycling conditions:
Initial Denaturation: 98°C for 30 seconds
25-35 cycles:
- 98°C for 10 seconds
- 55°C, 60°C, 65°C for 15 seconds
- 72°C for 15 seconds
Final Extension: 72°C for 5 minutes
<p>
