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Team:Nevada/Protocols
From 2012.igem.org
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<p>[[Team:Nevada/Notebook|Back to Notebook]] . [[Team:Nevada/Recipes|View Recipes]]<br/><br/> | <p>[[Team:Nevada/Notebook|Back to Notebook]] . [[Team:Nevada/Recipes|View Recipes]]<br/><br/> | ||
| - | <p>===Phusion PCR=== | + | <p> |
| + | ===Phusion PCR=== | ||
Thermocycling conditions:<br/> | Thermocycling conditions:<br/> | ||
Initial Denaturation: 98°C for 30 seconds <br/> | Initial Denaturation: 98°C for 30 seconds <br/> | ||
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Final Extension: 72°C for 5 minutes<br/> | Final Extension: 72°C for 5 minutes<br/> | ||
| - | <p>===Qiagen Miniprep kit=== | + | <p> |
| + | ===Qiagen Miniprep kit=== | ||
www.qiagen.com/hb/qiaprepminiprep<br/> | www.qiagen.com/hb/qiaprepminiprep<br/> | ||
Revision as of 19:14, 12 July 2012
Back to Notebook . View Recipes
<p>
Phusion PCR
Thermocycling conditions:
Initial Denaturation: 98°C for 30 seconds
25-35 cycles:
- 98°C for 10 seconds
- 55°C, 60°C, 65°C for 15 seconds
- 72°C for 15 seconds
Final Extension: 72°C for 5 minutes
<p>
