Team:RHIT

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Revision as of 19:24, 13 July 2012

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The goal of our project is to prepare yeast strains that can easily be used to determine the mating type of other yeast haploids. Our constructs will employ a promoter active in the pheromone response pathway and a latch-type circuit to produce a fluorescent reporter protein when the pathway is activated. We hope our constructs will facilitate the progress of myriad researchers who use yeast as a model organism.	File:RHIT team.png Your team picture
	Team RHIT

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-The purpose of the 2012 Rose-Hulman iGEM team’s project is to to construct mating type tester strains that can be used to determine the mating type of an unknown yeast strain. We aim to reduce the needed time to accomplish the test from the time required for the current method, which is approximately three days. In order to accomplish this, we will first establish two different tester strains of yeast, one of each mating type. These strains will be modified with a positive feedback loop, activated by the mating process, that continuously produces fluorescent protein. Each tester strain’s fluorescent protein will be a different color so that both a positive and negative response can be obtained from the test. We hope that this project will help to facilitate future breakthroughs in yeast genetics.
+The goal of our project is to prepare yeast strains that can easily be used to determine the mating type of other yeast haploids. Our constructs will employ a promoter active in the pheromone response pathway and a latch-type circuit to produce a fluorescent reporter protein when the pathway is activated. We hope our constructs will facilitate the progress of myriad researchers who use yeast as a model organism.
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