Team:Caltech/Notebook/Degradation

Degradation Notebook

June 22, 2012

Created a spreadsheet detailing various possible bacterial strains and plasmids to be used for complex polymer degradation
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June 25, 2012

Sent out requests for bacterial strains and plasmids based on spreadsheet
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June 26, 2012

Emailed Dr. Jared Leadbetter asking if he has strains + protocol for lignocellulose plates
Made carb. R plates
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June 27, 2012

Made minimal media agar plates, bottom layer of overlay plates
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June 28, 2012

Met with Professor Leadbetter to discuss difficulties with creating enrichment plates and finding bacterial samples
Will be following up on some of his suggestions

Notes from Meeting with Professor Jared Leadbetter from June 28th

Overlay Plate Options
- Top layer with minimal media, although either minimal media or just water + agarose should work
- Embed bacteria into top layer mix
- Use filter paper made of chosen carbon sources

Bacterial Strains
- Ponds around campus
- Beach
- Teredinibacter turnerae gen: helps degrade wood in shipworms (http://ijs.sgmjournals.org/content/52/6/2261.abstract)

May also choose a different host organism for transformation
- Instead of using E. coli can try using yeast (simplifies problem to only of degradation, and not also ethanol synthesis)
- Can also try bacteria found by Aztecs to make alcohol (Zymomonas mobilis)
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June 29, 2012

Emailed for samples of nitrocellulose, latex, and teflon membranes.
Researched Z. mobilis.
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July 2, 2012

Researched zymomonas mobilis, narrowed down focus to strain ZM4
Gathered more information on protocols specific to that strain.
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July 3, 2012

Looked up additional information about potential vectors to use to transform z. mobilis
Requested p42-0119 plasmid for z. mobilis from another lab
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July 5, 2012

Made 20% glucose solution stock and RM plates for Z. mobilis
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July 9, 2012

Ordered strains from the ATCC.
Discussed Biolog plates idea
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July 10, 2012

Research z mobilis degradation sugar targets
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July 11, 2012

Researched for genetic sequences for sugar degradation enzymes.
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