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Team:Caltech/Notebook/Degradation
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<br> Notes from Meeting with Professor Jared Leadbetter from June 28th | <br> Notes from Meeting with Professor Jared Leadbetter from June 28th | ||
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<br>Overlay Plate Options | <br>Overlay Plate Options | ||
<br>- Top layer with minimal media, although either minimal media or just water + agarose should work | <br>- Top layer with minimal media, although either minimal media or just water + agarose should work | ||
<br>- Embed bacteria into top layer mix | <br>- Embed bacteria into top layer mix | ||
<br>- Use filter paper made of chosen carbon sources | <br>- Use filter paper made of chosen carbon sources | ||
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<br>Bacterial Strains | <br>Bacterial Strains | ||
<br>- Ponds around campus | <br>- Ponds around campus | ||
<br>- Beach | <br>- Beach | ||
<br>- Teredinibacter turnerae gen: helps degrade wood in shipworms (http://ijs.sgmjournals.org/content/52/6/2261.abstract) | <br>- Teredinibacter turnerae gen: helps degrade wood in shipworms (http://ijs.sgmjournals.org/content/52/6/2261.abstract) | ||
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<br>May also choose a different host organism for transformation | <br>May also choose a different host organism for transformation | ||
<br>- Instead of using E. coli can try using yeast (simplifies problem to only of degradation, and not also ethanol synthesis) | <br>- Instead of using E. coli can try using yeast (simplifies problem to only of degradation, and not also ethanol synthesis) | ||
Revision as of 18:38, 11 July 2012
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Degradation Notebook
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