Team:TU-Eindhoven

From 2012.igem.org

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{{:Team:Cambridge/Templates/headerbar|colour=#386abc|title=The Project}}
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<div style="position:absolute; width:230px; left:5px; text-align:center;">A flask lit by E. coli transformed with one of our constructs </div>
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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<div style="position:absolute; width:230px; left:240px; text-align:center;">E. coli transformed with our different coloured bioluminescent systems</div>
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<div style="position:absolute; width:220px; left:485px; text-align:center;">Team members illuminated only by our bacteria</div>
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Over the course of the summer we built a set of BioBricks to allow bioluminescence in a wide range of colours which have applications both as reporters for [https://2010.igem.org/Team:Cambridge/Tools/microMeasure '''biosensors'''] and as [https://2010.igem.org/Team:Cambridge/Tools/Lighting '''natural light sources''']. We also developed software tools to aid construction of BioBrick parts and devices.
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{{:Team:Cambridge/Templates/Nolineheader2|header=Project Firefly}}
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We adopted a number of strategies to extend the use of '''firefly luciferase''':
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* [https://2010.igem.org/Team:Cambridge/Codons '''Codon optimisation'''] for increased light output
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* Use of a [https://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration '''luciferin regenerating enzyme'''].
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* Mutagenesis to create a number of [https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour '''different colours''']
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{{:Team:Cambridge/Templates/Nolineheader2|header=Project Vibrio
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We complemented these firefly systems, which require the addition of the substrate luciferin, with light producing systems from '''Vibrio fischeri'''.  We believe we have created the [https://2010.igem.org/Team:Cambridge/Bioluminescence/G28 '''first BioBrick'''] to emit light in normal E. coli strains without the addition of any external substrate.
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{{:Team:Cambridge/Templates/Nolineheader2|header=Tools}}
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During our project we made extensive use of [https://2010.igem.org/Team:Cambridge/Gibson/Introduction '''Gibson Assembly'''] to manufacture our parts, and have submitted an [https://2010.igem.org/Team:Cambridge/Gibson/RFC '''RFC'''] to the [http://bbf.openwetware.org/ '''BioBricks Foundation'''] to help future teams make best use of this technique.
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Along with this, we also constructed a number of tools to assist the synthetic biologists of the future:
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* [https://2010.igem.org/Team:Cambridge/Tools/Gibson '''Gibthon Construct Designer'''] allows the user to enter a series of BioBrick or GenBank IDs in a specific order and computes the appropriate primers for [https://2010.igem.org/Team:Cambridge/Gibson/Introduction '''Gibson Assembly'''].
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* [https://2010.igem.org/Team:Cambridge/Tools/GenBank '''BioBrick → GenBank'''] allows parts from the registry to be downloaded in .gb format, making them compatible with a wide range of biological software.
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* The [https://2010.igem.org/Team:Cambridge/Tools/Ligate '''Ligation Calculator'''] is a small calculator to help you work out the proportions to use for ligation in BioBrick assembly without having to worry about units.
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* The [https://2010.igem.org/Team:Cambridge/Tools/Eglometer '''E.glometer'''] is a cheap, easily built, piece of electronics for measuring bioluminescence.  It allows scientists without access to expensive plate readers to measure bacterial light output and has potential applications in [https://2010.igem.org/Team:Cambridge/Tools/microMeasure '''quantitative biosensors'''].
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{{:Team:Cambridge/Templates/Nolineheader2|header=Achievements in iGEM competition
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* Finalist
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|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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* Winner of "Best Wiki" award (awarded jointly to Cambridge and [https://2010.igem.org/Team:Imperial_College_London '''Imperial College London'''])
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|[[Image:TU-Eindhoven_logo.png|200px|right|frame]]
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* Winner of the iGEMers Prize (awarded jointly to five iGEM teams)
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* Awarded a Gold Medal
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Please see [http://https://igem.org/Results '''iGEM official results page'''] to see how all the teams did.
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:TU-Eindhoven_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:TU-Eindhoven | Team TU-Eindhoven]]
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* [http://www.newscientist.com/article/mg20827885.000-glowing-trees-could-light-up-city-streets.html New Scientist article]
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* [http://www.dailymail.co.uk/sciencetech/article-1333334/How-trees-glow-like-fireflies-day-replace-streetlights.html Daily Mail article]
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* [http://www.telegraph.co.uk/topics/christmas/8215302/The-science-of-Christmas-we-could-grow-our-own-fairy-lights-say-the-tree-wise-men.html The Telegraph Article]
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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[[Image:Cambridge_team_pictwo2010.jpg|center|frame|The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich,  Bill Collins]]
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!align="center"|[[Team:TU-Eindhoven|Home]]
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!align="center"|[[Team:TU-Eindhoven/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=TU-Eindhoven Official Team Profile]
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!align="center"|[[Team:TU-Eindhoven/Project|Project]]
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!align="center"|[[Team:TU-Eindhoven/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:TU-Eindhoven/Modeling|Modeling]]
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!align="center"|[[Team:TU-Eindhoven/Notebook|Notebook]]
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!align="center"|[[Team:TU-Eindhoven/Safety|Safety]]
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Revision as of 12:19, 11 July 2012

Team:Cambridge/Templates/headerMinimalprototype Team:Cambridge/Templates/boxesprototypenew

 

Team:Cambridge/Templates/headerbar

A flask lit by E. coli transformed with one of our constructs
E. coli transformed with our different coloured bioluminescent systems
Team members illuminated only by our bacteria


Over the course of the summer we built a set of BioBricks to allow bioluminescence in a wide range of colours which have applications both as reporters for biosensors and as natural light sources. We also developed software tools to aid construction of BioBrick parts and devices.

Team:Cambridge/Templates/Nolineheader2

We adopted a number of strategies to extend the use of firefly luciferase:

Team:Cambridge/Templates/Nolineheader2 We complemented these firefly systems, which require the addition of the substrate luciferin, with light producing systems from Vibrio fischeri. We believe we have created the first BioBrick to emit light in normal E. coli strains without the addition of any external substrate.

Team:Cambridge/Templates/Nolineheader2

During our project we made extensive use of Gibson Assembly to manufacture our parts, and have submitted an RFC to the [http://bbf.openwetware.org/ BioBricks Foundation] to help future teams make best use of this technique.

Along with this, we also constructed a number of tools to assist the synthetic biologists of the future:

  • Gibthon Construct Designer allows the user to enter a series of BioBrick or GenBank IDs in a specific order and computes the appropriate primers for Gibson Assembly.
  • BioBrick → GenBank allows parts from the registry to be downloaded in .gb format, making them compatible with a wide range of biological software.
  • The Ligation Calculator is a small calculator to help you work out the proportions to use for ligation in BioBrick assembly without having to worry about units.
  • The E.glometer is a cheap, easily built, piece of electronics for measuring bioluminescence. It allows scientists without access to expensive plate readers to measure bacterial light output and has potential applications in quantitative biosensors.

Team:Cambridge/Templates/Nolineheader2

  • Finalist
  • Winner of "Best Wiki" award (awarded jointly to Cambridge and Imperial College London)
  • Winner of the iGEMers Prize (awarded jointly to five iGEM teams)
  • Awarded a Gold Medal

Please see [http://https://igem.org/Results iGEM official results page] to see how all the teams did.

Team:Cambridge/Templates/Nolineheader2

  • [http://www.newscientist.com/article/mg20827885.000-glowing-trees-could-light-up-city-streets.html New Scientist article]
  • [http://www.dailymail.co.uk/sciencetech/article-1333334/How-trees-glow-like-fireflies-day-replace-streetlights.html Daily Mail article]
  • [http://www.telegraph.co.uk/topics/christmas/8215302/The-science-of-Christmas-we-could-grow-our-own-fairy-lights-say-the-tree-wise-men.html The Telegraph Article]
File:Cambridge team pictwo2010.jpg
The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich, Bill Collins

Team:Cambridge/Templates/footerMinimal

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