Team:WashU/Week7

From 2012.igem.org

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==Saffron in a Kan==
==Saffron in a Kan==
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Today, we maxi-prepped our construct CS42S, digested both CS42S and our plasmid PSL2131, and ran them both on gels.  
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Today, we maxi-prepped our construct CS42S, digested both CS42S and our plasmid PSL2131, and ran them both on gels. We made one-well gels in order to allow us to gel purify the constructs and recover DNA.
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[PICTURES BELOW]
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https://lh4.googleusercontent.com/-iur9dT0upCs/T_xkV8J0wEI/AAAAAAAAASc/Y4m5RhWVvao/s640/construct%2520extraction.jpg
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Then we gel purified the construct and plasmid and nanodropped them. [TABLE OF NANODROP RESULTS]
Then we gel purified the construct and plasmid and nanodropped them. [TABLE OF NANODROP RESULTS]

Revision as of 17:28, 10 July 2012



Monday, July 9

Saffron in a Kan

Today, we maxi-prepped our construct CS42S, digested both CS42S and our plasmid PSL2131, and ran them both on gels. We made one-well gels in order to allow us to gel purify the constructs and recover DNA. construct%2520extraction.jpg

Then we gel purified the construct and plasmid and nanodropped them. [TABLE OF NANODROP RESULTS]


Tuesday, July 10

Ligation of construct and plasmid Transformation with the ligation results


Wednesday, July 11


Thursday, July 12


Friday, July 13

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