Team:Tec-Monterrey/antifreeze/project

From 2012.igem.org

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.content #cl6{ background:url(https://static.igem.org/mediawiki/2012/4/4a/TECMTY_Antifreeze_notebook_normal.png) no-repeat; height:110px; width:100px; border:none;}
.content #cl6{ background:url(https://static.igem.org/mediawiki/2012/4/4a/TECMTY_Antifreeze_notebook_normal.png) no-repeat; height:110px; width:100px; border:none;}
.content #cl6:hover{ background:url(https://static.igem.org/mediawiki/2012/2/24/TECMTY_Antifreeze_notebook_hover.png) no-repeat;}
.content #cl6:hover{ background:url(https://static.igem.org/mediawiki/2012/2/24/TECMTY_Antifreeze_notebook_hover.png) no-repeat;}
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#div_subcontent #slideshow {
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width:520px;
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margin-left: auto ;
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margin-right: auto ;
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position:relative;
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#div_subcontent #slideshow #slidesContainer {
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margin:0 auto;
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width:520px;
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height:290px;
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overflow:auto;
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}
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#div_subcontent #slideshow #slidesContainer .slide {
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margin:0 auto;
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width:500px;
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height:290px;
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}
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#div_subcontent .control {
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display:block;
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width:40px;
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height:290px;
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text-indent:-10000px;
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cursor: pointer;
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position:absolute;
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top:0;
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#div_subcontent #leftControl {
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background:transparent url("https://static.igem.org/mediawiki/igem.org/f/fd/TECMTY_Bgaleria_allergen_normal.png") no-repeat center;
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#div_subcontent #rightControl {
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background:transparent url("https://static.igem.org/mediawiki/igem.org/6/69/TECMTY_Bgaleria_antifreeze_normal.png") no-repeat center;
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.tabcolor {
.tabcolor {
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$("#div_subcontent").fadeOut(1000);
$("#div_subcontent").fadeOut(1000);
});
});
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//Tabs
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$('#tab1').click(function() {
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$('#replace').html($('#content1').html());
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});
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$('#tab2').click(function() {
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$('#replace').html($('#content2').html());
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prepareSlides();
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});
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//Slides
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function prepareSlides() {
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var currentPos = 0;
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var sWidth = 520;
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var slides = $('div#div_subcontent .slide');
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var numSlides = slides.length;
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$('div#div_subcontent #slidesContainer').css('overflow','hidden');
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slides.wrapAll('<div id="slideInner"></div>');
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slides.css({
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'float':'left',
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'width':sWidth
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});
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$('div#div_subcontent #slideInner').css('width',sWidth*numSlides);
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$('div#div_subcontent #slideshow').prepend('<span class="control" id="leftControl">Move left</span>');
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$('div#div_subcontent #slideshow').append('<span class="control" id="rightControl">Move right</span>');
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manageControls(currentPos,numSlides);
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$('div#div_subcontent .control').bind('click', function() {
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manageControls(currentPos,numSlides);
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$('div#div_subcontent #slideInner').animate({
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'marginLeft' : sWidth*(-currentPos)
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function manageControls(position, numSlides) {
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else { $('div#div_subcontent #leftControl').show(); }
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else { $('div#div_subcontent #rightControl').show(); }
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<img src="https://static.igem.org/mediawiki/2012/f/fd/TECMTY_Antifreeze_results_hover.png" alt="11" />
<img src="https://static.igem.org/mediawiki/2012/f/fd/TECMTY_Antifreeze_results_hover.png" alt="11" />
<img src="https://static.igem.org/mediawiki/2012/2/24/TECMTY_Antifreeze_notebook_hover.png" alt="12" />
<img src="https://static.igem.org/mediawiki/2012/2/24/TECMTY_Antifreeze_notebook_hover.png" alt="12" />
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</span>
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<span class="preload" id="content1">
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<p>This project's objective is the development of a new E. coli strain capable of surviving numerous steps of freezing and thawing, due to the expression of antifreeze proteins from the beetle Rhagium inquisitor (RiAFP). We expect this new strain to be easier to handle in research labs and to increase the overall efficiency of transformed cells.</p>
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<p>We plan to modify this strain's chromosomal DNA by Red/ET homologous recombination, and to insert the necessary sequences for the expression of the RiAFP in the bacteria's cytoplasmic and periplasmic spaces. Furthermore, we will use an experimental design to find out the best storing conditions for this strain during cryopreservation.</p>
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</span>
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<span class="preload" id="content2">
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<div id="slideshow">
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<div id="slidesContainer">
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<div class="slide">
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<img src="img/1.jpg" alt="Slide1" />
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</div>
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<div class="slide">
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<img src="img/1.jpg" alt="Slide2" />
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</div>
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<div class="slide">
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<img src="img/1.jpg" alt="Slide3" />
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</div>
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<div class="slide">
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<img src="img/1.jpg" alt="Slide4" />
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</div>
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<div class="slide">
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<img src="img/1.jpg" alt="Slide5" />
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</div>
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</div>
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</div>
</span>
</span>

Revision as of 20:36, 10 July 2012

Tec Igem 2012 1 2 3 4 5 6 7 8 9 10 11 12

This project's objective is the development of a new E. coli strain capable of surviving numerous steps of freezing and thawing, due to the expression of antifreeze proteins from the beetle Rhagium inquisitor (RiAFP). We expect this new strain to be easier to handle in research labs and to increase the overall efficiency of transformed cells.

We plan to modify this strain's chromosomal DNA by Red/ET homologous recombination, and to insert the necessary sequences for the expression of the RiAFP in the bacteria's cytoplasmic and periplasmic spaces. Furthermore, we will use an experimental design to find out the best storing conditions for this strain during cryopreservation.

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project

This project's objective is the development of a new E. coli strain capable of surviving numerous steps of freezing and thawing, due to the expression of antifreeze proteins from the beetle Rhagium inquisitor (RiAFP). We expect this new strain to be easier to handle in research labs and to increase the overall efficiency of transformed cells.

We plan to modify this strain's chromosomal DNA by Red/ET homologous recombination, and to insert the necessary sequences for the expression of the RiAFP in the bacteria's cytoplasmic and periplasmic spaces. Furthermore, we will use an experimental design to find out the best storing conditions for this strain during cryopreservation.

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