Team:Lyon-INSA/datapage

From 2012.igem.org

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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802003" target="_blank"><font color="#664499"><b>2. Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b>BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in <i>E. coli</i> and Erythromycin resistant in <i>B. subtilis</i>. It is a <b>low</b> copy number plasmid in <i>B. subtilis</i> and a high copy number plasmid in <i>E. coli</i>. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in <i>B. subtilis</i> by a transformation yield of 70 transformants/µg and a erythromycin resistance up to 900 µg/mL.
 
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802000" target="_blank"><font color="#664499"><b>3. Main Page</b></font></a>: <b>Lysostaphin generator for <i>B. subtilis</i></b> BBa_K802000  : This part associates the <i>Bacillus subtilis</i> constitutive promoter (P<sub>veg</sub>) with the lysostaphin gene. This part allows an efficient killing of <i>S. aureus</i> cells.<br><br>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802000" target="_blank"><font color="#664499"><b>2. Main Page</b></font></a>: <b>Lysostaphin generator for <i>B. subtilis</i></b> BBa_K802000  : This part associates the <i>Bacillus subtilis</i> constitutive promoter (P<sub>veg</sub>) with the lysostaphin gene. This part allows an efficient killing of <i>S. aureus</i> cells.<br><br>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802009" target="_blank"><font color="#664499"><b>4. Main Page</b></font></a>: <b>Surfactin generator and biofilm repressor for <i>B. subtilis</i></b> BBa_K802009: This part can be used to induce surfactin production and to repress the biofilm formation in <i> B. subtilis</i> strains (COAT module).  
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802009" target="_blank"><font color="#664499"><b>3. Main Page</b></font></a>: <b>Surfactin generator and biofilm repressor for <i>B. subtilis</i></b> BBa_K802009: This part can be used to induce surfactin production and to repress the biofilm formation in <i> B. subtilis</i> strains (COAT module).  
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<div class="petitSsTitre">We've also characterized the following new parts </div>
<div class="petitSsTitre">We've also characterized the following new parts </div>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802003" target="_blank"><font color="#664499"><b> Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b>BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in <i>E. coli</i> and Erythromycin resistant in <i>B. subtilis</i>. It is a <b>low</b> copy number plasmid in <i>B. subtilis</i> and a high copy number plasmid in <i>E. coli</i>. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in <i>B. subtilis</i> by a transformation yield of 70 transformants/µg and a erythromycin resistance up to 900 µg/mL.
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802002" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>P<sub>lac</sub>-RBS-GFP</b>  BBa_K802002 : This part has been designed to determine the behavior of the P<sub>lac</sub> promoter used to drive the STICK module (<span class="unProto" onclick="window.open('http://partsregistry.org/Part:BBa_K802009', 'Part BBa_K802009'); return false;">Part BBa_K802009</span>)
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802002" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>P<sub>lac</sub>-RBS-GFP</b>  BBa_K802002 : This part has been designed to determine the behavior of the P<sub>lac</sub> promoter used to drive the STICK module (<span class="unProto" onclick="window.open('http://partsregistry.org/Part:BBa_K802009', 'Part BBa_K802009'); return false;">Part BBa_K802009</span>)
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802004" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b> BBa_K802004 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in <i>E. coli</i> and Erythromycin resistant in <i>B. subtilis</i>. It is a <b>high</b> copy number plasmid in both <i>B. subtilis</i> and <i>E. coli</i>. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in <i>B. subtilis</i> by a transformation yield of 80 transformants/µg and a erythromycin resistance to at least 1.5 mg/mL.
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802004" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: <b>Shuttle vector for <i>E. coli</i> and <i>B. subtilis</i> </b> BBa_K802004 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in <i>E. coli</i> and Erythromycin resistant in <i>B. subtilis</i>. It is a <b>high</b> copy number plasmid in both <i>B. subtilis</i> and <i>E. coli</i>. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in <i>B. subtilis</i> by a transformation yield of 80 transformants/µg and a erythromycin resistance to at least 1.5 mg/mL.
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802007" target="_blank"><font color="#664499"><b>Main Page</b></font></a>: This part can be used to impede biofilm formation in B. subtilis, more particularly if it is arbB-. This part was characterized in conjuction with parts K802006 and K802008</br></br>
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Revision as of 16:18, 26 October 2012

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Our parts :

Data about our favorite new parts
1. Main Page: Dispersin generator for B. subtilis BBa_K802001 : This part associates the Bacillus subtilis constitutive promoter (Pveg) with the dispersin B gene (dspB). This part allows to efficiently scatter Staphylococci biofilms.



2. Main Page: Lysostaphin generator for B. subtilis BBa_K802000 : This part associates the Bacillus subtilis constitutive promoter (Pveg) with the lysostaphin gene. This part allows an efficient killing of S. aureus cells.

3. Main Page: Surfactin generator and biofilm repressor for B. subtilis BBa_K802009: This part can be used to induce surfactin production and to repress the biofilm formation in B. subtilis strains (COAT module).
We've also characterized the following new parts
Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a low copy number plasmid in B. subtilis and a high copy number plasmid in E. coli. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in B. subtilis by a transformation yield of 70 transformants/µg and a erythromycin resistance up to 900 µg/mL.

Main Page: Plac-RBS-GFP BBa_K802002 : This part has been designed to determine the behavior of the Plac promoter used to drive the STICK module (Part BBa_K802009)

Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802004 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high copy number plasmid in both B. subtilis and E. coli. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in B. subtilis by a transformation yield of 80 transformants/µg and a erythromycin resistance to at least 1.5 mg/mL.

Main Page: This part can be used to impede biofilm formation in B. subtilis, more particularly if it is arbB-. This part was characterized in conjuction with parts K802006 and K802008


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