Team:Potsdam Bioware/Lab/Labjournal/October

From 2012.igem.org

(Difference between revisions)
(→2012-10-12)
(→2012-10-12)
Line 46: Line 46:
===<p style="background-color: rgb(240, 20, 70);">2012-10-12</p>===
===<p style="background-color: rgb(240, 20, 70);">2012-10-12</p>===
 +
 +
 +
<p style="background-color: rgb(238, 221, 130); font-weight:bold;">cell culture </p>
 +
<br>
 +
<b>Investigator:</b>Kerstin<br>
 +
<br>
 +
<b>Topic: </b>cell-culture <br>
 +
<br>
 +
* passaging of CHO w Zeocin
 +
* passaging of HT1080
 +
* passaging of HEK AAV 293
 +
* passaging of HeLa
 +
 +
 +
===<p style="background-color: rgb(240, 20, 70);">2012-10-15</p>===
 +
 +
 +
<p style="background-color: rgb(238, 221, 130); font-weight:bold;">cell culture </p>
 +
<br>
 +
<b>Investigator:</b>Kerstin<br>
 +
<br>
 +
<b>Topic: </b>cell-culture <br>
 +
<br>
 +
* passaging of CHO w Zeocin
 +
* passaging of HT1080
 +
* passaging of HEK AAV 293
 +
* passaging of HeLa
 +
 +
 +
===<p style="background-color: rgb(240, 20, 70);">2012-10-17</p>===
 +
 +
 +
<p style="background-color: rgb(238, 221, 130); font-weight:bold;">cell culture </p>
 +
<br>
 +
<b>Investigator:</b>Kerstin<br>
 +
<br>
 +
<b>Topic: </b>cell-culture <br>
 +
<br>
 +
* passaging of CHO w Zeocin
 +
* passaging of HT1080
 +
* passaging of HEK AAV 293
 +
* passaging of HeLa
 +
 +
 +
===<p style="background-color: rgb(240, 20, 70);">2012-10-19</p>===
 +
 +
 +
<p style="background-color: rgb(238, 221, 130); font-weight:bold;">cell culture </p>
 +
<br>
 +
<b>Investigator:</b>Kerstin<br>
 +
<br>
 +
<b>Topic: </b>cell-culture <br>
 +
<br>
 +
* passaging of CHO w Zeocin
 +
* passaging of HT1080
 +
* passaging of HEK AAV 293
 +
* passaging of HeLa
 +
 +
 +
===<p style="background-color: rgb(240, 20, 70);">2012-10-22</p>===
 +
 +
 +
<p style="background-color: rgb(238, 221, 130); font-weight:bold;">cell culture </p>
 +
<br>
 +
<b>Investigator:</b>Kerstin<br>
 +
<br>
 +
<b>Topic: </b>cell-culture <br>
 +
<br>
 +
* passaging of CHO w Zeocin
 +
* passaging of HT1080
 +
* passaging of HEK AAV 293
 +
* passaging of HeLa
 +
 +
 +
===<p style="background-color: rgb(240, 20, 70);">2012-10-23</p>===
 +
 +
 +
<p style="background-color: rgb(238, 221, 130); font-weight:bold;">cell culture </p>
 +
<br>
 +
<b>Investigator:</b>Kerstin<br>
 +
<br>
 +
<b>Topic: </b>cell-culture <br>
 +
<br>
 +
* passaging of CHO w Zeocin
 +
* passaging of HT1080
 +
* passaging of HEK AAV 293
 +
* passaging of HeLa
 +
 +
 +
===<p style="background-color: rgb(240, 20, 70);">2012-10-24</p>===
 +
 +
 +
<p style="background-color: rgb(238, 221, 130); font-weight:bold;">cell culture </p>
 +
<br>
 +
<b>Investigator:</b>Kerstin<br>
 +
<br>
 +
<b>Topic: </b>cell-culture <br>
 +
<br>
 +
* passaging of CHO w Zeocin
 +
* passaging of HT1080
 +
* passaging of HEK AAV 293
 +
* passaging of HeLa
 +
 +
===<p style="background-color: rgb(240, 20, 70);">2012-10-25</p>===

Revision as of 13:40, 25 October 2012


Contents

AID

2012-10-10

Inoculation of plasmid samples of the 48h retransformation plates (after FACS)

Investigators: Tom


Time: 2012-10-10


Materials:

  • LB medium
  • Amp stock solution
  • plate with cultures:

EGFR-C-AID without NES, with NLS+Kozak sequence+eGFP

(after FACS selection)

Method:

Inoculation of:

5 cultures per plate in 5 ml LB medium + 5µL Amp.

(--> 5 cultures)

Further tasks:

  • Miniprep


Antibody

2012-10-12

cell culture


Investigator:Kerstin

Topic: cell-culture

  • passaging of CHO w Zeocin
  • passaging of HT1080
  • passaging of HEK AAV 293
  • passaging of HeLa


2012-10-15

cell culture


Investigator:Kerstin

Topic: cell-culture

  • passaging of CHO w Zeocin
  • passaging of HT1080
  • passaging of HEK AAV 293
  • passaging of HeLa


2012-10-17

cell culture


Investigator:Kerstin

Topic: cell-culture

  • passaging of CHO w Zeocin
  • passaging of HT1080
  • passaging of HEK AAV 293
  • passaging of HeLa


2012-10-19

cell culture


Investigator:Kerstin

Topic: cell-culture

  • passaging of CHO w Zeocin
  • passaging of HT1080
  • passaging of HEK AAV 293
  • passaging of HeLa


2012-10-22

cell culture


Investigator:Kerstin

Topic: cell-culture

  • passaging of CHO w Zeocin
  • passaging of HT1080
  • passaging of HEK AAV 293
  • passaging of HeLa


2012-10-23

cell culture


Investigator:Kerstin

Topic: cell-culture

  • passaging of CHO w Zeocin
  • passaging of HT1080
  • passaging of HEK AAV 293
  • passaging of HeLa


2012-10-24

cell culture


Investigator:Kerstin

Topic: cell-culture

  • passaging of CHO w Zeocin
  • passaging of HT1080
  • passaging of HEK AAV 293
  • passaging of HeLa

2012-10-25

cell culture


Investigator:Kerstin

Topic: cell-culture

  • passaging of CHO w Zeocin
  • passaging of HT1080
  • passaging of HEK AAV 293
  • passaging of HeLa

Virus

XXX

XXX

Investigator: XXX

Aim: XXX

Materials:

XXX

Method:

XXX

Results:

XXX

Further tasks

XXX
Retrieved from "http://2012.igem.org/Team:Potsdam_Bioware/Lab/Labjournal/October"