Team:Potsdam Bioware/Lab/Labjournal/October

From 2012.igem.org

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<b>Topic: </b>cell-culture <br>
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* passaging of CHO w Zeocin
* passaging of HT1080
* passaging of HT1080
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* dilution of stably transfected cells (Nanobody construct clone 29, originally from transfected Well 3) to 1-2 cells per 150µl and seeding in 4x 96-Well plates
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* passaging of HEK AAV 293
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* seeding of stably transfected CHO Clone 29 cells in Ibidi Dish
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* passaging of HeLa
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* change of medium in 75cm² flasks with stably transfected cells (Clone 4 and 29)
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* harvest cells for Western Blot (CHO not transfected, CHO stably transfected Clone 4 and 29)
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==Virus==
==Virus==

Revision as of 13:38, 25 October 2012


Contents

AID

2012-10-10

Inoculation of plasmid samples of the 48h retransformation plates (after FACS)

Investigators: Tom


Time: 2012-10-10


Materials:

  • LB medium
  • Amp stock solution
  • plate with cultures:

EGFR-C-AID without NES, with NLS+Kozak sequence+eGFP

(after FACS selection)

Method:

Inoculation of:

5 cultures per plate in 5 ml LB medium + 5µL Amp.

(--> 5 cultures)

Further tasks:

  • Miniprep


Antibody

2012-10-12

cell culture


Investigator:Kerstin

Topic: cell-culture

  • passaging of CHO w Zeocin
  • passaging of HT1080
  • passaging of HEK AAV 293
  • passaging of HeLa

Virus

XXX

XXX

Investigator: XXX

Aim: XXX

Materials:

XXX

Method:

XXX

Results:

XXX

Further tasks

XXX
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