Team:Leicester/Project

From 2012.igem.org

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The first part involves halving the expanded polystyrene (EPS) in our returned citizen science experiment (CSE) kits so that we'll still have some of each sample left should we need it. First we need to do a practice with unused EPS strips (control and test). Pour/pipette 20ml LB agar into a petri dish and allow to set. Put line down middle in permanent marker USING RULER! swab whole half strip of EPS and spread onto the left half of the agar (or side labeled as 'swab'). Try to swab any mud off the strip if possible. Then place the half strip in the centre of the other half labeled 'strip'. Leave for a week at room tewmperature on the lab bench and examine agar for colonies. The other half of the strip should be put back into cold storage for future use.
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The first part involves halving the expanded polystyrene lengthways (EPS) with sterilized scissors in our returned citizen science experiment (CSE) kits so that we'll still have some of each sample left should we need it. First we need to do a practice with unused EPS strips (control and test). Pour/pipette 20ml LB agar into a petri dish and allow to set. Put line down middle in permanent marker USING RULER! Add PBS to the half strip of EPS and scrape with spreader, before spreading onto the left half of the agar (or side labeled as 'swab'). Try to swab any mud off the strip if possible. Then place the half strip in the centre of the other half labeled 'strip'. Leave for a week at room temperature on the lab bench and examine agar for colonies. The other half of the strip should be put back into cold storage for future use.
--[[User:Lt114|Luke Thompson]] 07:51, 9 July 2012 (CDT)
--[[User:Lt114|Luke Thompson]] 07:51, 9 July 2012 (CDT)

Revision as of 13:15, 9 July 2012


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Contents

Overall project

Our project is about trying to reduce the waste going to landfill by engineering a bacteria to degrade polystyrene.

Project Details

Part 2

The Experiments

Plating out the returned Citizen Science Experiment kits

The first part involves halving the expanded polystyrene lengthways (EPS) with sterilized scissors in our returned citizen science experiment (CSE) kits so that we'll still have some of each sample left should we need it. First we need to do a practice with unused EPS strips (control and test). Pour/pipette 20ml LB agar into a petri dish and allow to set. Put line down middle in permanent marker USING RULER! Add PBS to the half strip of EPS and scrape with spreader, before spreading onto the left half of the agar (or side labeled as 'swab'). Try to swab any mud off the strip if possible. Then place the half strip in the centre of the other half labeled 'strip'. Leave for a week at room temperature on the lab bench and examine agar for colonies. The other half of the strip should be put back into cold storage for future use.

--Luke Thompson 07:51, 9 July 2012 (CDT)

Part 3

Results

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