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Team:Tianjin/Data
From 2012.igem.org
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| + | =Agarose Gel Electrophoresis= | ||
| + | [[file:TJU12-data13.JPG|thumb|left|7.14 t7 rbs' crtE]][[file:TJU12-data14.JPG|thumb|none|7.31 16s' RFP-GFP AMP]] | ||
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| + | [[file:TJU12-data1.JPG|thumb|left|8.03 pSB1C3 K3 Digestion]][[file:TJU12-data2.JPG|thumb|none|8.16 16s' mutate to remove EcoRI]] | ||
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| + | [[file:TJU12-data3.JPG|thumb|left|8.17 16S' pSB1C3]][[file:TJU12-data4.JPG|thumb|none|8.17 Mutate the RBS of Amp using PYES2]] | ||
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| + | [[file:TJU12-data5.JPG|thumb|left|8.17 Yeast Assembly using pSB3C5R & pSB3K3]][[file:TJU12-data6.JPG|thumb|none|8.18 pBad-16S' remove EcorI, PSB1C3]] | ||
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| + | [[file:TJU12-data7.JPG|thumb|left|9.2 6I 6G Gel extraction and verification]][[file:TJU12-data8.JPG|thumb|none|9.2 6I,6G P16S Gel extraction and verification]] | ||
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| + | [[file:TJU12-data9.JPG|thumb|left|9.2 P16S digestion]][[file:TJU12-data10.JPG|thumb|none|9.16 yeast cPCR 1]] | ||
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| + | [[file:TJU12-data11.JPG|thumb|left|9.16 yeast cPCR 2]][[file:TJU12-data12.JPG|thumb|none|8.01 16s GFP & RFP]] | ||
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Latest revision as of 13:30, 24 October 2012
BioBrick
Favorite Tianjin 2012 iGEM Team Parts
| Name | Type | Description | Designer | Length | ||
|---|---|---|---|---|---|---|
| 1 | W | BBa_K821001 | Reporter | RFP Reporter with o-RBS | Qinfeng Wu, Bin Jia | 1059 |
| 2 | W | BBa_K821002 | Coding | Orthogonal 16S DNA sequence from rrnB | Qinfeng Wu, Bin Jia | 1894 |
| 3 | W | BBa_K821012 | Generator | PBad(promoter)+orthogonal 16S RNA | Jinlai Zhang, Dongchang Qin | 3226 |
Tianjin 2012 iGEM Team Parts Sandbox
| Name | Type | Description | Designer | Length | ||
|---|---|---|---|---|---|---|
| 1 | W | BBa_K821003 | Coding | 16S DNA sequence from rrnB | Jinlai Zhang, Dongchang Qin | 1894 |
| 2 | W | BBa_K821004 | Regulatory | PBad Promoter | Jinlai Zhang, Dongchang Qin | 1324 |
| 3 | W | BBa_K821005 | Reporter | CmR label from pKD3 | Qinfeng Wu, Bin Jia | 1014 |
| 4 | W | BBa_K821006 | Project | KanR label from pKD3 | Jinlai Zhang, Dongchang Qin | 1304 |
| 5 | W | BBa_K821007 | Composite | PL(promoter)+orthogonal 16S RNA | Qinfeng Wu, Bin Jia | 1957 |
| 6 | W | BBa_K821008 | Composite | PLtet+orthogonal 16S RNA | Jinlai Zhang, Dongchang Qin | 1956 |
| 7 | W | BBa_K821009 | RBS | Orthogonal RBS 1 | Qinfeng Wu, Bin Jia | 6 |
| 8 | W | BBa_K821010 | Composite | PLacI+Native RBS+RFP+Native RBS+GFP+Terminator | Qinfeng Wu, Bin Jia | 1833 |
| 9 | W | BBa_K821003 | Composite | PLacI+Orthogonal RBS+RFP+Native RBS+GFP+Terminator | Jinlai Zhang, Dongchang Qin | 1827 |
Agarose Gel Electrophoresis
