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Team/CINVESTAV-IPN-UNAM MX/home.htm
From 2012.igem.org
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| - | <h1><em>Rhodofactory | + | <h1><em>Rhodofactory, controlling genetic expression: an oxygen and light response!</em></h1> |
| - | + | <img src="https://static.igem.org/mediawiki/2012/e/ec/Mascota.jpg" width="329" height="327" class="left"> | |
| - | + | <p>The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, | |
| - | + | darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking | |
| - | + | advantage of this, our project aims to build two genetic control systems based on R. sphaeroides | |
| - | + | photosynthesis cluster regulation. The first one is a light dependent system controlled by two | |
| - | + | proteins AppA/PpsR that works like an antirepresor/repressor mechanism, and the second one is | |
| - | + | an oxygen dependent system of two-component called PrrA/PrrB. This two devices were tested on | |
| - | + | R. palustris chassis, using a cassete in which a reporter (GFP) is regulated by external conditions | |
| - | + | that activate or repress its expresion. Once we have characterized the functionality of these | |
| - | + | networks, our perspective is to develop a Rhodofactory, it means to control the produccion of | |
| - | + | differents metabolites, such as biodiesel and butanol, using simple signals.</p> | |
| - | + | ||
<div style="clear: both;"> </div> | <div style="clear: both;"> </div> | ||
</div> | </div> | ||
Revision as of 02:40, 24 October 2012
Rhodofactory, controlling genetic expression: an oxygen and light response!
The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking advantage of this, our project aims to build two genetic control systems based on R. sphaeroides photosynthesis cluster regulation. The first one is a light dependent system controlled by two proteins AppA/PpsR that works like an antirepresor/repressor mechanism, and the second one is an oxygen dependent system of two-component called PrrA/PrrB. This two devices were tested on R. palustris chassis, using a cassete in which a reporter (GFP) is regulated by external conditions that activate or repress its expresion. Once we have characterized the functionality of these networks, our perspective is to develop a Rhodofactory, it means to control the produccion of differents metabolites, such as biodiesel and butanol, using simple signals.
Rhodofactory 2012
