Team:Johns Hopkins-Wetware/Parts
From 2012.igem.org
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- | Using the conversion vectors described above, we BioBricked five ethanol-inducible yeast promoters | + | Using the conversion vectors described above, we BioBricked five ethanol-inducible yeast promoters (<a href="http://partsregistry.org/Part:BBa_K799001">BBa_K799001</a>,<a href="http://partsregistry.org/Part:BBa_K799003">BBa_K799003</a>,<a href="http://partsregistry.org/Part:BBa_K799005">BBa_K799005</a>, |
- | + | <a href="http://partsregistry.org/Part:BBa_K799007">BBa_K799007</a>, | |
- | + | <a href="http://partsregistry.org/Part:BBa_K799009">BBa_K799009</a>), human cytochrome p450 2E1 (CYP2E1) | |
- | + | <a href="http://partsregistry.org/Part:BBa_K799027">BBa_K799027</a> The yeast terminator from the MFA2 gene (<a href="http://partsregistry.org/Part:BBa_K799029">BBa_K799029</a> | |
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- | + | We also BioBricked a GFP sequence lacking BsaI and BsmBI(<a href="http://partsregistry.org/Part:BBa_K799028">BBa_K799028</a>)that we constructed for use in our Yeast Golden Gate Assembly method. We have sequence verified all of these parts. The ethanol-inducible promoters have been characterized through GFP expression under various ethanol levels. We have tested the ability of CYP2E1 to reduce ethanol concentration over a 24 hour fermentation. | |
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- | The yeast terminator from the MFA2 gene | + | |
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- | We also BioBricked a GFP sequence lacking BsaI and BsmBI | + | |
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- | that we constructed for use in our Yeast Golden Gate Assembly method. We have sequence verified all of these parts. The ethanol-inducible promoters have been characterized through GFP expression under various ethanol levels. We have tested the ability of CYP2E1 to reduce ethanol concentration over a 24 hour fermentation. | + | |
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