Team:MIT/ResultsBiobricks

From 2012.igem.org

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<h1>Our Favorite BioBricks</h1>
<h1>Our Favorite BioBricks</h1>
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<h2>BBa_K779500: Reporter top strand with bulge</h2>
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<h2>BBa_K779500: Reporter top strand</h2>
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<a href="http://partsregistry.org/Part:BBa_K779500">Registry entry</a>
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One half of our reporter RNA complex.  When this RNA strand is displaced from its bottom strand, the RQ fluorescence quencher on this strand separates from a ROX fluorescent molecule on the bottom strand, causing the bottom strand to fluoresce.  We arrived at this design after numerous iterations over the course of the summer.
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Characterization on <a href="https://2012.igem.org/Team:MIT/ResultsFoundational#SDbio">Foundational page</a>.
<h2>BBa_K779607: Tre-Tight-mKate inducible fluorescence with DOX</h2>
<h2>BBa_K779607: Tre-Tight-mKate inducible fluorescence with DOX</h2>

Revision as of 02:41, 4 October 2012

DEPRECATED. DO NOT USE OR EDIT. If a page uses this template, relink with MIT-results2.

Our Favorite BioBricks

BBa_K779500: Reporter top strand

Registry entry
One half of our reporter RNA complex. When this RNA strand is displaced from its bottom strand, the RQ fluorescence quencher on this strand separates from a ROX fluorescent molecule on the bottom strand, causing the bottom strand to fluoresce. We arrived at this design after numerous iterations over the course of the summer.

Characterization on Foundational page.

BBa_K779607: Tre-Tight-mKate inducible fluorescence with DOX

BBa_K779400: U6-TetO-FF1 inducible RNA expression

All BioBricks

NameTypeDescriptionDesignerLength
BBa_K779100DNAFuel 1 - mRNA domain 1 DNA sensorDivya Israni18
BBa_K779101DNAGate 1 - mRNA domain 1 DNA sensorDivya Israni25
BBa_K779102DNAOutput 1 - mRNA domain 1 DNA sensorDivya Israni33
BBa_K779103DNAInput Mimic 1 - mRNA domain 1 DNA sensorDivya Israni20
BBa_K779104DNAFuel 2 - mRNA domain 2 DNA sensorDivya Israni18
BBa_K779105DNAGate 2 - mRNA domain 2 DNA sensorDivya Israni25
BBa_K779106DNAOutput 2 - mRNA domain 2 DNA sensorDivya Israni33
BBa_K779107DNAInput Mimic 2 - mRNA domain 2 DNA sensorDivya Israni20
BBa_K779108DNAFuel 3 - mRNA domain 3 DNA sensorDivya Israni18
BBa_K779109DNAGate 3 - mRNA domain 3 DNA sensorDivya Israni25
BBa_K779110DNAOutput 3 - mRNA domain 3 DNA sensorDivya Israni33
BBa_K779111DNAInput Mimic 3 - mRNA domain 3 DNA sensorDivya Israni20
BBa_K779112DNAFuel 4 - mRNA domain 4 DNA sensorDivya Israni18
BBa_K779113DNAGate 4 - mRNA domain 4 DNA sensorDivya Israni33
BBa_K779114DNAOutput 4 - mRNA domain 4 DNA sensorDivya Israni25
BBa_K779115DNAInput Mimic 4 - mRNA domain 4 DNA sensorDivya Israni20
BBa_K779116RNAShort RNA reporter bottom strand (with ROX fluorophore) Divya Israni20
BBa_K779117RNARNA Reporter top strand with quencher (RQ)Divya Israni15
BBa_K779118RNARNA Input S6Divya Israni20
BBa_K779119RNARNA Control input (scrambled)Divya Israni20
BBa_K779120RNARNA Reporter top strand with quencher (RQ) and tag fluorophore (Alexa 488)Divya Israni15
BBa_K779121DNAShort DNA reporter top strand (with RQ quencher)Divya Israni15
BBa_K779122DNAShort DNA reporter bottom strand (with ROX fluorophore)Divya Israni20
BBa_K779123DNAa - In vitro NOT gateDivya Israni43
BBa_K779124DNAb1 - In vitro NOT gateDivya Israni28
BBa_K779125DNAb2 - In vitro NOT gateDivya Israni20
BBa_K779126DNAc1 - In vitro NOT gateDivya Israni43
BBa_K779127DNAc2 - In vitro NOT gateDivya Israni29
BBa_K779128DNAc3 - In vitro NOT gateDivya Israni47
BBa_K779129DNAd1 - In vitro NOT gateDivya Israni29
BBa_K779130DNAd2 - In vitro NOT gateDivya Israni38
BBa_K779131DNAe - In vitro NOT gateDivya Israni34
BBa_K779200RegulatoryHef1AGiulio Alighieri1174
BBa_K779201RegulatoryCMVGiulio Alighieri70
BBa_K779202RegulatoryTRE-TightGiulio Alighieri196
BBa_K779300ReportereBFP2Giulio Alighieri726
BBa_K779301ReportereYFPGiulio Alighieri726
BBa_K779302ReportereYFP-4xFF1Giulio Alighieri859
BBa_K779303ReportereYFP-4xFF4Giulio Alighieri835
BBa_K779304ReportermKateGiulio Alighieri721
BBa_K779305CodingrtTA3Giulio Alighieri714
BBa_K779306ReporterTagBFPKatie Bodner699
BBa_K779307ReporterTagBFP-FF5Katie Bodner801
BBa_K779308CodingTetRKatie Bodner630
BBa_K779309ReportereBFP2-1xKatie Bodner754
BBa_K779310ReportereBFP2-2xKatie Bodner778
BBa_K779311ReporterHH-mKate - Hammerhead attached to front of mKateKatie Bodner802
BBa_K779312ReportermKate-HH - Hammerhead attached behind mKateKatie Bodner804
BBa_K779313ReporterHHM-mKate - Repressible hammerhead attached to front of mKateFelix Sun838
BBa_K779314ReportermKate-HHm - Repressible hammerhead behind mKateFelix Sun844
BBa_K779315Coding5' Hammerhead with loss-of-function mutationFelix Sun190
BBa_K779316Coding3' Hammerhead with loss-of-function mutationFelix Sun186
BBa_K779317Coding5' HammerheadFelix Sun190
BBa_K779318Coding3' HammerheadFelix Sun186
BBa_K779400RegulatoryU6-TetO-FF1Divya Israni326
BBa_K779401RegulatoryU6-TetO-S6Divya Israni277
BBa_K779402RegulatoryU6-TetO-S1Divya Israni277
BBa_K779403RegulatoryU6-TetO-FF4Divya Israni596
BBa_K779404RegulatoryU6-TetO-DecoyFF4-3pDivya Israni287
BBa_K779405RegulatoryU6-TetO-DecoyFF4-3p-4ntinDivya Israni291
BBa_K779406RegulatoryU6-TetO-TuDFF4-3pDivya Israni390
BBa_K779407RegulatoryU6-TetO-TuDFF4-3p-4ntinDivya Israni398
BBa_K779408RegulatoryU6-TetO-SaDivya Israni285
BBa_K779409RegulatoryU6-TetO-ScDivya Israni285
BBa_K779500OtherLong reporter top strand with bulgeKristjan Eerik Kaseniit24
BBa_K779501OtherLong reporter top strandKristjan Eerik Kaseniit20
BBa_K779502OtherLong reporter bottom strandKristjan Eerik Kaseniit28
BBa_K779503OtherLong input strandKristjan Eerik Kaseniit28
BBa_K779504OtherLong scrambled input strandKristjan Eerik Kaseniit28
BBa_K779600GeneratorHef1a-eYFPGiulio Alighieri1922
BBa_K779601GeneratorHef1a-mKateGiulio Alighieri1917
BBa_K779602GeneratorHef1a-TagBFP-FF5Giulio Alighieri1997
BBa_K779603GeneratorHef1a-eYFP-4xFF1Giulio Alighieri1922
BBa_K779604GeneratorHef1a-rTTA3Giulio Alighieri1926
BBa_K779605GeneratorHef1A-TetRKatie Bodner1842
BBa_K779606GeneratorTre-T-eYFP Katie Bodner944
BBa_K779607GeneratorTre-Tight-mKateKatie Bodner939

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