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UTK Knoxville- October
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| + | 9/21/12 | ||
| + | Colony PCR Confirmed: | ||
| + | J63010 +Adh + mOrange + pIRES + GFP + cyc | ||
| + | J63010 +Adh + mOrange + pIRES2 + GFP + cyc | ||
| + | J63010 +Adh + mOrange + pSAP + GFP + cyc | ||
| + | |||
| + | 9/23/12 | ||
| + | Colony PCR Confirmed: | ||
| + | J63010 + Adh + Neo | ||
| + | J63010 +Adh + mOrange + YAP1 + GFP + cyc | ||
| + | J63010 +Adh + mOrange + URE2 + GFP + cyc | ||
| + | J63010 +Adh + mOrange + HAP4 + GFP + cyc | ||
| + | |||
| + | |||
| + | 9/24/12 | ||
| + | Digestions: | ||
| + | J63010 + pIRES + GFP + cyc : SpeI and PstI | ||
| + | J63010 + pIRES2 + GFP + cyc : SpeI and PstI | ||
| + | J63010 + pSAP + GFP + cyc : SpeI and PstI | ||
| + | J63010 + Adh + Neo : XbaI and PstI | ||
| + | J63010 +Adh + mOrange + pIRES + GFP + cyc + Adh + Neo : EcoRI and PstI | ||
| + | J63010 + Adh + mOrange +pIRES2 + GFP + cyc + Adh + Neo: EcoRI and PstI | ||
| + | J63010 + Adh + mOrange +pSAP + GFP + cyc + Adh + Neo: EcoRI and PstI | ||
| + | J63010 + Adh + mOrange + cyc : SpeI and PstI | ||
| + | J63010 + Adh + GFP + cyc: SpeI and PstI | ||
| + | |||
| + | |||
| + | 9/25/12 | ||
| + | Ligations performed to create constructs: | ||
| + | J63010 + Adh + mOrange + cyc + Adh + Neo | ||
| + | J63010 + Adh + GFP + cyc + Adh + Neo | ||
| + | J63010 + pIRES + GFP + cyc + Adh + Neo | ||
| + | J63010 + pIRES2 + GFP + cyc + Adh + Neo | ||
| + | J63010 + pSAP + GFP + cyc + Adh + Neo | ||
| + | |||
| + | 9/28/12 | ||
| + | Colony PCR Confirmed: | ||
| + | J63010 + Adh + mOrange + cyc + Adh + Neo (AOC) | ||
| + | J63010 + Adh + GFP + cyc + Adh + Neo (AGC) | ||
| + | J63010 + pIRES + GFP + cyc + Adh + Neo (pIRES control) | ||
| + | J63010 + pIRES2 + GFP + cyc + Adh + Neo (pIRES2 control) | ||
| + | J63010 + pSAP + GFP + cyc + Adh + Neo (pSAP control) | ||
| + | |||
| + | BY4741 Yeast electroporated with: | ||
| + | AOC, AGC, pIRES control, pIRES2 control, pSAP control,pIRES complete, pIRES2 complete, pSAP complete. | ||
| + | Electroporations were plated onto 200ug/ml G418 YPD plates. | ||
| + | |||
| + | 10/1/12 | ||
| + | Colonies finally appeared on plates. Colonies were picked into corresponding 200ug/ml G418 YPD plates. No colonies grew in culture. | ||
Revision as of 03:18, 4 October 2012
