Team:Purdue/Parts

From 2012.igem.org

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<h1> Parts Registry </h1>
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<h5> An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will place them in the http://partsregistry.org Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the http://partsregistry.org Registry, not on your team wiki.
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<td> Name</td><td>Description</td><td>Registry link</td><td>Part type</td><td>Availability</td></tr>
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<p> Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for  users who wish to know more.
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<td>precA_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/c/c0/BBa_K862000.PNG"/><br/>
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This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E.coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a RecA promoter (<a href="http://partsregistry.org/Part:BBa_J22106" class="external text" title="http://partsregistry.org/Part:BBa_J22106 rel="nofollow">BBa_J22106</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>).</td><td>
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<table border="1">
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<p>
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   <caption>Parts Submitted</caption>
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   <a href="http://partsregistry.org/Part:BBa_K862000">BBa_K862000</a>  
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    <th>Name</th>
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    <th>Description</th>
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    <th>Registry Link</th>
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    <th>Part Type</th>
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    <th>Availability</th>
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  </tr>
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  <tr>
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    <td>Curli Construct</td>
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    <td>Adhesion Protein Activator</td>
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    <td> To be completed </td>
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    <td> To be completed </td>
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    <td> To be completed </td>
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  </tr>
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    <td>OmpA - Sililcatein Alpha</td>
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    <td> Transmembrane protein with attached Silica Binding Protein (Fusion Protein)</td>
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    <td> To be completed </td>
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    <td> To be completed </td>
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    <td> To be completed </td>
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  </tr>
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</td><td> Measurement</td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr>
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<td>psulA_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/4/4f/BBa_K862001.PNG"/><br/>
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<br/>
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This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E.coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a SulA promoter (<a href="http://partsregistry.org/Part:BBa_K518010" class="external text" title="http://partsregistry.org/Part:BBa_K518010 rel="nofollow">BBa_K518010</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>). <td><a href="http://partsregistry.org/Part:BBa_K862001" class="external text" title="http://partsregistry.org/Part:BBa_K862001 rel="nofollow">BBa_K862001</a></td><td> Measurement
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</td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr>
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<tr>
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<td>precB_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/a/a8/BBa_K862002.PNG"/><br/>
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<br/>
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This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E. coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a RecB promoter (<a href="http://partsregistry.org/Part:BBa_K862003" class="external text" title="http://partsregistry.org/Part:BBa_K862003 rel="nofollow">BBa_K862003</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>).</td><td><a href="http://partsregistry.org/Part:BBa_K862002" class="external text" title="http://partsregistry.org/Part:BBa_K862002" rel="nofollow">BBa_K862002</a></td><td> Measurement
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</td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr>
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</td></tr>
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<tr>
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<td>precB</td><td><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png"/><br/>
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The RecB promoter sequence was obtained from the <i>E.coli</i> MG1655 genome sequence (<a href="http://ecoliwiki.net/colipedia/index.php/recB:Gene">http://ecoliwiki.net/colipedia/index.php/recB:Gene</a>). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis.
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We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI predigested reporter part.
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<br>
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<br/>RecB_fw: 5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA</br>CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3' <br>
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<br>RecB_rev: 5'-ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAAC</br>GCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg-3'</td><td><a href="http://partsregistry.org/Part:BBa_K862003" class="external text" title="http://partsregistry.org/Part:BBa_K862003" rel="nofollow">BBa_K862003</a></td><td> Regulatory
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</td><td>can be cloned on oligo basis (sequence provided)</td></tr>
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</table>
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<groupparts>iGEM012 Purdue</groupparts>
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Revision as of 02:42, 7 July 2012

Parts Registry

NameDescriptionRegistry linkPart typeAvailability precA_LacZ

This is a part for the precise quantification of UV-radiation or radioactive radiation in E.coli (recA+) strains, i.e. BL21(DE3). It consists of a RecA promoter (BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (BBa_K173004).

BBa_K862000

Measurement parts registry psulA_LacZ

This is a part for the precise quantification of UV-radiation or radioactive radiation in E.coli (recA+) strains, i.e. BL21(DE3). It consists of a SulA promoter (BBa_K518010) fused to a LacZ reporter cloned in front of a double terminator (BBa_K173004). BBa_K862001 Measurement parts registry precB_LacZ

This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consists of a RecB promoter (BBa_K862003) fused to a LacZ reporter cloned in front of a double terminator (BBa_K173004).BBa_K862002 Measurement parts registry precB

The RecB promoter sequence was obtained from the E.coli MG1655 genome sequence (http://ecoliwiki.net/colipedia/index.php/recB:Gene). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis. We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI predigested reporter part.

RecB_fw: 5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA
CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3'

RecB_rev: 5'-ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAAC
GCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg-3'BBa_K862003 Regulatory can be cloned on oligo basis (sequence provided)

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