Team:UT Dallas/pop3 o

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Latest revision as of 02:17, 4 October 2012

Three Population Cascade Overview

Our team worked to create an three population signal propagation mechanism with three different strains of bacteria. Each of these populations uses a different quorum sensing molecule to promote one strain while repressing the other. The promoted strain then fluoresces and sends out another quorum sensing molecule that promotes next strain. This process continues with each strain, thus creating an oscillator.

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 <h2 class='title' style='font-size: 120%;'>Three Population Cascade Overview</h2>
-An oscillator is a system that repeatedly goes back and forth about a specific level of expression in order to achieve an equilibrium. Many think of an oscillator as being a physical system such as a pendulum or a clock, but oscillators are also repeatedly found in biological systems. Biological oscillators are characterized by a positive and negative feedback loop.<br>
+Our team worked to create an three population signal propagation mechanism with three different strains of bacteria. Each of these populations uses a different quorum sensing molecule to promote one strain while repressing the other. The promoted strain then fluoresces and sends out another quorum sensing molecule that promotes next strain. This process continues with each strain, thus creating an oscillator.
-For our oscillator, we created three different strains using E.coli bacteria. These strains used three different quorum sensing molecules acyl homoserine-lactone (AHL), (autoinducer-1) AI-1, and (autoinducer-2) AI-2 coupled with yellow, red, and cyan fluorescent proteins to create a visual oscillating effect.