Team:Carnegie Mellon/Met-Challenges

From 2012.igem.org

(Difference between revisions)
Line 18: Line 18:
</li>
</li>
<li>
<li>
-
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Hom-Safety">Safety</a>
+
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Hom-Acknowledgements">Acknowledgements</a>
</li>
</li>
</ul>
</ul>
Line 31: Line 31:
<li>
<li>
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Bio-Submitted">Submitted Parts</a>
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Bio-Submitted">Submitted Parts</a>
-
</li>
 
-
<li>
 
-
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Bio-Properties">Properties</a>
 
</li>
</li>
</ul>
</ul>
</li>
</li>
-
<li class="current" style ='width: 193px'>
+
<li class="current" style ='width: 193px'>
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Met-Overview">Methods and Results</a>
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Met-Overview">Methods and Results</a>
<ul>
<ul>
Line 56: Line 53:
<li>
<li>
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Met-Notebook">Notebook</a>
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Met-Notebook">Notebook</a>
 +
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Met-Safety">Safety</a>
 +
</li>
</ul>
</ul>
</li>
</li>
Line 78: Line 79:
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Overview">Human Practices</a>
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Overview">Human Practices</a>
<ul>
<ul>
-
<li class = 'offset' style ='width: 687px'> <a href="#"></a></li>
+
<li class = 'offset' style ='width: 622px'> <a href="#"></a></li>
<li>
<li>
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Overview">Overview</a>
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Overview">Overview</a>
Line 87: Line 88:
<li>
<li>
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Circuit">Circuit Kit</a>
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Circuit">Circuit Kit</a>
-
<ul>
+
</li>
-
<li>
+
<li>
-
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Documentation">Documentation</a>
+
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Software">Software</a>
-
</li>
+
-
<li>
+
-
<a href="https://2012.igem.org/Team:Carnegie_Mellon/Hum-Software">Software</a>
+
-
</li>
+
-
</ul>
+
</li>
</li>
</ul>
</ul>

Revision as of 14:07, 2 October 2012

Image:CMU_image6.jpeg




  • Cloning procedures did not work initially due to confounding factors of contamination in media and competent cells, inappropriate design of digestion sites, and non-optimal PCR reactions.
  • We have attempted to clone our constructs into pSB1C3 and another plasmid vector that has both EcoR1 and Pst1 digestion sites. The cloning using the plasmid vector worked in the first trial. However, the cloning using pSB1C3 was more difficult than we expected and only worked after four trials and extensive optimization of our protocols. We have not been able to pinpoint the problems
  • Image:TartanFooter.jpeg