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Team:IvyTech-South Bend/Project
From 2012.igem.org
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=== Part 2 === | === Part 2 === | ||
| - | + | Not wanting apparent success to get in our way we hypothesized that the addition of a second ArsR gene under pArs control should further raise the threshold of the device. We therefore added a terminiator to J33201 and coupled it upstream from J33201 and as a control K190015. A down stream cut of K190015 from J661002 expression plasmid yields pArs+RBS+RFP+Term. | |
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=== The Experiments === | === The Experiments === | ||
Revision as of 16:22, 2 October 2012
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Part 2
Not wanting apparent success to get in our way we hypothesized that the addition of a second ArsR gene under pArs control should further raise the threshold of the device. We therefore added a terminiator to J33201 and coupled it upstream from J33201 and as a control K190015. A down stream cut of K190015 from J661002 expression plasmid yields pArs+RBS+RFP+Term.
