Revision as of 17:01, 1 October 2012

Biofuels and Bacterial Animations

The Caltech 2012 iGEM team's project focused on manipulating bacteria to degrade stable organic polymers such as lignin and alginate; using these substrates to synthesize biofuels, specifically ethanol; and directing their ATP synthesis mechanism to rely on proteorhodopsin as the source of the proton gradient, freeing NADH to interact in the synthetic pathway. Caltech is also collaborating with California Institute of the Arts to create an animation using a plate of E. coli transformed with mCherry plasmid.

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-Caltech’s 2012 iGEM <a href="https://2012.igem.org/Team:Caltech/Team">team</a> aims to manipulate bacteria to <a href="https://2012.igem.org/Team:Caltech/Project#Degradation_Project">degrade</a> stable organic polymers such as lignin and alginate; to use these substrates to <a href="https://2012.igem.org/Team:Caltech/Project#Biofuel_Project">synthesize biofuels</a>, specifically ethanol; and to direct their ATP synthesis mechanism to rely on <a href="https://2012.igem.org/Team:Caltech/Project#Proteorhodopsin_Project">proteorhodopsin</a> as the source of the proton gradient, freeing NADH to interact in the synthetic pathway instead.  We will develop the three facets of the project in different strains of E. coli.  We will then splice the manipulated sequences into one new organism.
+The Caltech 2012 iGEM <a href="https://2012.igem.org/Team:Caltech/Team">team's</a> project focused on manipulating bacteria to <a href="https://2012.igem.org/Team:Caltech/Project#Degradation_Project">degrade</a> stable organic polymers such as lignin and alginate; using these substrates to <a href="https://2012.igem.org/Team:Caltech/Project#Biofuel_Project">synthesize biofuels</a>, specifically ethanol; and directing their ATP synthesis mechanism to rely on <a href="https://2012.igem.org/Team:Caltech/Project#Proteorhodopsin_Project">proteorhodopsin</a> as the source of the proton gradient, freeing NADH to interact in the synthetic pathway.  Caltech is also collaborating with California Institute of the Arts to create an animation using a plate of E. coli transformed with mCherry plasmid.
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 <img src="https://static.igem.org/mediawiki/2012/c/ca/Caltech_Flowchart.png" alt="Project Flowchart"/>
 <img src="https://static.igem.org/mediawiki/2012/b/be/Coliroid.png">
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-Caltech is also collaborating with California Institute of the Arts to create an animation using a plate of E. coli transformed with mcherry plasmid.
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