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Team:Evry/Notebook/July/2
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Revision as of 09:02, 9 July 2012
Today's achievements:
1) T10 bacteria transformation with DNA ligated on Friday (29/06)- protocol as before
2) Transformed bacteria spreaded on Petri's plate- LB-agar-ampiciline- incubation 37 degrees Celsius, overnight
3) SOC preparation:
1,4g Hanahan's Broth
0,18g Glucose
50mL MiliQ water
filtrated on microfilter (if you have more important volume, autoclave it)
