Team:Cornell/Notebook/Nah operon

From 2012.igem.org

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===[[Team:Cornell/Notebook/Nah_operon/July15|July 15th-21st]]===
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===June 24th-30th===
 
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====June 27th, Wednesday====
 
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[[Image:2012_627_RafDylanGelMaking.jpg|thumb|right|Rafael and Dylan making an agarose gel.]]
 
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*Ran digest of gibson-assembled nah operon with [[gel electrophoresis]]
 
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====June 28th, Thursday====
 

Revision as of 21:14, 3 July 2012

Home Team Project Parts Modeling Notebook Protocols Safety Attributions

Contents

The purpose of this subproject is to make the nah operon biobrick compatible for the use of future iGEM teams. The nah operon codes for a pathway that converts naphthalene into salicylate. In our system, this will allow naphthalene to be detected by the salicylate reporter. There are three standard cut sites internal to the operon which we are removing - PstI, and two NotI sites. This biobrick compatible piece could be useful to future teams in projects that involve bioremediation or detection of naphthalene.

June

June 10th-16th

June 17th-23rd

June 24th-30th

July

July 1st-7th

July 8th-14th

===July 15th-21st===

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