Team:Colombia/Parts

From 2012.igem.org

(Difference between revisions)
m (Alert System:)
m (Ralstonia:)
Line 25: Line 25:
-
'''pchS:'''
+
'''pchS:'''  
'''pchR:'''
'''pchR:'''
-
'''pchA:'''
+
'''pchA:'''LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.
'''PxpcR:'''
'''PxpcR:'''

Revision as of 02:56, 27 September 2012

Template:Https://2012.igem.org/User:Tabima

Contents

Parts

Toxin- Antitoxin (TA) Modules

Our Basic Parts

TA BioBricks1-2.png

Our Composite Parts

TA BioBricks2-2.png

The links to our BioBricks are

Ralstonia:

Ralstoniaparts.png


pchS:


pchR:

pchA:LysR-type transcriptional regulator that controls expression of many genes and is induced in a density manner, relying on the unusual Quorum Sensing signal 3OH-PAME.

PxpcR:

Aliivibrio fischeri

Alis.png

The overall model can be seen in figures in the main page of Aliivibrio/Streptomyces.

ChiP: [http://www.ncbi.nlm.nih.gov/gene/3279194 Chitoporin] is a specific channel that allows N-acetylglucosamine dimers to cross the outer membrane to the periplasmic space,

ChiA: [http://www.ncbi.nlm.nih.gov/gene/3277358 Chitinase] is an hydrolase that cleaves the glycosidic bonds on the chitin polymer.

ChiS: [http://www.ncbi.nlm.nih.gov/gene/3279287 The histidine-kinase sensor] is part of a two-component signalling system, that activates a promoter, in this case pChitin.

CBP: [http://www.ncbi.nlm.nih.gov/gene/3279341 Chitin-binding protein] is able to bind the N-acetyl glucosamine dimers in the periplasmic space. The complex CBP+(GlcNAc)2 serves as an activator of the signalling from chiS to the promoter.

pChitin: This 100bp region upstream of chiP is thought to be activated by the activation of chiS.

Alert System:

Cutandpasteparts.png