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Team:Amsterdam/project/biobricks
From 2012.igem.org
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<h2>Favourite Constructs</h2> | <h2>Favourite Constructs</h2> | ||
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| + | <h4>IPTG inducible expression of M.ScaI methyltransferase<br>(IPTG -> M.ScaI) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K874100 BBa_K874100]</h4> | ||
<h4>IPTG inducible expression of M.ScaI methyltransferase<br>(IPTG -> M.ScaI) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K874100 BBa_K874100]</h4> | <h4>IPTG inducible expression of M.ScaI methyltransferase<br>(IPTG -> M.ScaI) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K874100 BBa_K874100]</h4> | ||
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<h4>Arabinose inducible expression of M.ScaI methyltransferase<br> (ARA -> M.ScaI) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K874101 BBa_K874101]</h4> | <h4>Arabinose inducible expression of M.ScaI methyltransferase<br> (ARA -> M.ScaI) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K874101 BBa_K874101]</h4> | ||
<p>After the assessment of the first BioBrick we deemed it nessesary to change te promotor to the pBAD (Arabinose) promoter. This therefore is still the first proof of concept but contains a different <b>Reader</b> as described in our molecular design. This BioBrick was also extensively studied so for an in dept view of the experiments we performed using this BioBrick you can look at the [[Team:Amsterdam/data/experimental |Experimental Setup]] section. We managed to insert this BioBrick in both the pSB1AT3 and pSB1C3 backbones but all testing was done in pSB1AT3.</p> | <p>After the assessment of the first BioBrick we deemed it nessesary to change te promotor to the pBAD (Arabinose) promoter. This therefore is still the first proof of concept but contains a different <b>Reader</b> as described in our molecular design. This BioBrick was also extensively studied so for an in dept view of the experiments we performed using this BioBrick you can look at the [[Team:Amsterdam/data/experimental |Experimental Setup]] section. We managed to insert this BioBrick in both the pSB1AT3 and pSB1C3 backbones but all testing was done in pSB1AT3.</p> | ||
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Revision as of 02:27, 27 September 2012
BioBricks
<groupparts>iGEM12 Amsterdam</groupparts>
Favourite Constructs
IPTG inducible expression of M.ScaI methyltransferase
(IPTG -> M.ScaI) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K874100 BBa_K874100]
IPTG inducible expression of M.ScaI methyltransferase
(IPTG -> M.ScaI) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K874100 BBa_K874100]
This BioBrick contains the first proof of concept and contains the both the Reader and the Sensor described in our molecular design. Therefore this is also the most extensively studied BioBrick in our project, for an in dept view of the experiments we performed using this BioBrick you can look at the Experimental Setup section. We managed to insert this BioBrick in both the pSB1AT3 and pSB1C3 backbones but all testing was done in pSB1AT3.
Arabinose inducible expression of M.ScaI methyltransferase
(ARA -> M.ScaI) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K874101 BBa_K874101]
After the assessment of the first BioBrick we deemed it nessesary to change te promotor to the pBAD (Arabinose) promoter. This therefore is still the first proof of concept but contains a different Reader as described in our molecular design. This BioBrick was also extensively studied so for an in dept view of the experiments we performed using this BioBrick you can look at the Experimental Setup section. We managed to insert this BioBrick in both the pSB1AT3 and pSB1C3 backbones but all testing was done in pSB1AT3.
