Team:Georgia Tech

From 2012.igem.org

(Difference between revisions)
(Prototype team page)
Line 24: Line 24:
|-
|-
|
|
-
''Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
+
''Currently, scientific endeavors are being pursued to engineer a synthetic quorum sensing system in E. Coli to express green fluorescent protein (GFP) in response to exogenous quorum sensing molecules. However, certain limitation exists as regards to the time required for the expression of GFP fluorescence. Anywhere from thirty minutes to two hours is necessary from the addition of the autoinducer until sufficient GFP can be transcribed, translated, accumulated, and detected, which for some synthetic biology applications may be unacceptable.
 +
 
 +
To this end, the goal of our experiment is to develop an alternative method to quickly visualize the response of E. coli to exogenous autoinducer by expressing the already present fusion proteins of GFP to the autoinducer receptor. Specifically, the C-terminus end of the GFP protein would be fused to one copy of the receptor gene which will be TraR from Agrobacterium tumefaciens, with the N-terminus end fused to a second copy of the same receptor. The GFP/receptor domain will get continuously transcribed and translated in the bacteria but it will not be able to fluoresce as the domains do not constitute a functional GFP protein. This will be brought about by the dimerization of the TraR receptor in the presence of autoinducers. This alternative method of inducing GFP expression is hypothesized to be efficient and quicker than the methods currently used as it bypasses the transcription and translation machinery of the cell while expressing the GFP.
 +
''
|[[Image:Georgia_Tech_team.png|right|frame|Your team picture]]
|[[Image:Georgia_Tech_team.png|right|frame|Your team picture]]
|-
|-

Revision as of 16:32, 15 July 2012


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace.



You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.

Currently, scientific endeavors are being pursued to engineer a synthetic quorum sensing system in E. Coli to express green fluorescent protein (GFP) in response to exogenous quorum sensing molecules. However, certain limitation exists as regards to the time required for the expression of GFP fluorescence. Anywhere from thirty minutes to two hours is necessary from the addition of the autoinducer until sufficient GFP can be transcribed, translated, accumulated, and detected, which for some synthetic biology applications may be unacceptable.

To this end, the goal of our experiment is to develop an alternative method to quickly visualize the response of E. coli to exogenous autoinducer by expressing the already present fusion proteins of GFP to the autoinducer receptor. Specifically, the C-terminus end of the GFP protein would be fused to one copy of the receptor gene which will be TraR from Agrobacterium tumefaciens, with the N-terminus end fused to a second copy of the same receptor. The GFP/receptor domain will get continuously transcribed and translated in the bacteria but it will not be able to fluoresce as the domains do not constitute a functional GFP protein. This will be brought about by the dimerization of the TraR receptor in the presence of autoinducers. This alternative method of inducing GFP expression is hypothesized to be efficient and quicker than the methods currently used as it bypasses the transcription and translation machinery of the cell while expressing the GFP.

File:Georgia Tech team.png
Your team picture
Team Georgia_Tech


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions