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Team:SDU-Denmark/labwork/Notebook/week4
From 2012.igem.org
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<p> <b>23-07-2012 to 29-07-2012</b> </p> | <p> <b>23-07-2012 to 29-07-2012</b> </p> | ||
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| - | Another checkdigest was made on SST colonies, we had our doubts about the entegrity of the formerly used restriction enzyme, so a newer | + | Another checkdigest was made on SST colonies, we had our doubts about the entegrity of the formerly used restriction enzyme, so a newer EcoRI was used, a get was made and this time we got results. After a checkdigest that showed bands at expected lengths and nanodrop, two samples of 1-SST was sent for sequencing.<br/><br/> |
| - | EcoRI was used, a get was made and this time we got results. After a checkdigest that showed bands at expected lengths and nanodrop, two | + | |
| - | samples of 1-SST was sent for sequencing. | + | |
| - | 4 days later we recieved results from sequencing. As we expected from the original ncbi sequences, the genes contained illegal restriction sites. | + | 4 days later we recieved results from sequencing. As we expected from the original ncbi sequences, the genes contained illegal restriction sites.<br/> |
| - | 1-SST contained 1 SpeI restriction site and 1-FFT had 1 EcoRI and 1 XbaI. | + | 1-SST contained 1 SpeI restriction site and 1-FFT had 1 EcoRI and 1 XbaI. <br/><br/> |
| - | Primers for mutagenesis were constructed and ordered with the help of our supervisor Steffen | + | Primers for mutagenesis were constructed and ordered with the help of our supervisor Steffen Schmidt |
</p> | </p> | ||
Latest revision as of 02:07, 27 September 2012
Laboratory Notebook
23-07-2012 to 29-07-2012
Another checkdigest was made on SST colonies, we had our doubts about the entegrity of the formerly used restriction enzyme, so a newer EcoRI was used, a get was made and this time we got results. After a checkdigest that showed bands at expected lengths and nanodrop, two samples of 1-SST was sent for sequencing.
4 days later we recieved results from sequencing. As we expected from the original ncbi sequences, the genes contained illegal restriction sites.
1-SST contained 1 SpeI restriction site and 1-FFT had 1 EcoRI and 1 XbaI.
Primers for mutagenesis were constructed and ordered with the help of our supervisor Steffen Schmidt
