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Team:SEU A/Experiment/proof1
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<span>Experiment Notebook.</span> | <span>Experiment Notebook.</span> | ||
</header> | </header> | ||
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<a href="https://2012.igem.org/Team:SEU_A/Experiment/proof1"><img src='https://static.igem.org/mediawiki/2012/3/3f/Seua_exp_HSP.png' width='200' height='200'></img></a> | <a href="https://2012.igem.org/Team:SEU_A/Experiment/proof1"><img src='https://static.igem.org/mediawiki/2012/3/3f/Seua_exp_HSP.png' width='200' height='200'></img></a> | ||
<a href="https://2012.igem.org/Team:SEU_A/Experiment/proof2"><img src='https://static.igem.org/mediawiki/2012/1/1f/Seua_exp_sweet_gene.png' width='200' height='200'></img></a> | <a href="https://2012.igem.org/Team:SEU_A/Experiment/proof2"><img src='https://static.igem.org/mediawiki/2012/1/1f/Seua_exp_sweet_gene.png' width='200' height='200'></img></a> | ||
<a href="https://2012.igem.org/Team:SEU_A/Experiment/proof3"><img src='https://static.igem.org/mediawiki/2012/5/5b/Seua_exp_conjugation.png' width='200' height='200'></img></a> | <a href="https://2012.igem.org/Team:SEU_A/Experiment/proof3"><img src='https://static.igem.org/mediawiki/2012/5/5b/Seua_exp_conjugation.png' width='200' height='200'></img></a> | ||
<a href="https://2012.igem.org/Team:SEU_A/Experiment/proof4"><img src='https://static.igem.org/mediawiki/2012/8/8a/Seua_exp_death.png' width='200' height='200'></img></a> | <a href="https://2012.igem.org/Team:SEU_A/Experiment/proof4"><img src='https://static.igem.org/mediawiki/2012/8/8a/Seua_exp_death.png' width='200' height='200'></img></a> | ||
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| - | + | <h2>The confirmation experiment of HSP promoter</h2></br> | |
| + | We use K873012(HSP promoter + E0240) which we construct by combining k873002 and E0240 to test the function of HSP promoter . The functions we expect from this kit are as follows: at normal temperature（e.g. 37℃）, the E. Coli do not express the protein; when the temperature rises to a certain degree (such as 42 ℃), the transcription will be activated, expressing green fluorescent protein.</br></br> | ||
| + | <center><img src='https://static.igem.org/mediawiki/2012/a/a5/Seua_exp_proof1_HSP1.png'></img></center> | ||
| + | <strong>Experimental procedure:</strong></br> | ||
| + | Step 1: recover K873012,and shake it in 37℃ for 12 to16h at150rpm.</br> | ||
| + | Step 2: use 50ul bacterial liquid which has been diluted times to coat sagar Luria-Bertani medium. Set temperature and induced factor (IPTG, 1 mM) control.</br> | ||
| + | <center><img src='https://static.igem.org/mediawiki/2012/c/c8/Seua_exp_proof_HSP2.png'></img></center> | ||
| + | Fig1. The experimental procedure of HSP promoter’s confirmation experiment | ||
| + | </br> | ||
</div> | </div> | ||
Revision as of 02:10, 27 September 2012
Diary - Part II
Experiment Notebook.
The confirmation experiment of HSP promoter
We use K873012(HSP promoter + E0240) which we construct by combining k873002 and E0240 to test the function of HSP promoter . The functions we expect from this kit are as follows: at normal temperature（e.g. 37℃）, the E. Coli do not express the protein; when the temperature rises to a certain degree (such as 42 ℃), the transcription will be activated, expressing green fluorescent protein.
Biomedical Engineer School, SEU | iGEM 2012
Copyright © Southeast University iGEM 2012 Team A, All rights reserved.
