Team:SUSTC-Shenzhen-B

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                     <li><a href="http://www.terminatorefficiency.com/"><h3>TTEC Web Tool</h3></a></li>
                     <li><a href="http://www.terminatorefficiency.com/"><h3>TTEC Web Tool</h3></a></li>
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                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment"><h3>User Comments</h3></a></li>
                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment"><h3>User Comments</h3></a></li>
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Revision as of 04:01, 27 September 2012

Template:SUSTC-B1 - 2012.igem.org

Template:SUSTC-B1

From 2012.igem.org

Title

Romantic Getaway

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Family Getaway

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Suite Getaway

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Welcome

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Nam eget justo velit. Vestibulum dignissim vestibulum volutpat. Morbi quam odio, facilisis ut scelerisque id, pretium blandit diam. Suspendisse quam magna, sollicitudin vitae volutpat et, porttitor ut sem. Mauris nec dolor erat. Vestibulum condimentum libero ac elit rutrum pharetra. Nunc aliquet nisi et erat vulputate pellentesque.

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1

Design Ideas

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2

Problem Areas

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3

Green Garden

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4

Planting Areas

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SUSTC iGEM Theme - Free CSS Template

Transcriptional Terminator Efficiency Calculator (TTEC)

We studied the rho-independent transcription terminators which regulate gene expression using both theoretical model and experiment method.

We first developed a theoretical model. This model calculated the free energy of RNA folding and could predict the secondary structure of terminators. From the secondary structure, we proposed an algorithm that can predict terminator efficiency.

To validate our theoretical prediction, we also perform wetlab experiments. We construct 100 terminators. We measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators.

We also developed a software and a web server for people to calculate their terminators and built a database of terminator efficiency which we believe to be the largest database of such kind. Our work is by far the most comprehensive study on terminator efficiency.

Have a Try !

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