Team:WashU/Protocols/GlycerolStock

From 2012.igem.org

(Difference between revisions)
(Created page with "__NOTOC__ {{WashUback}} =General Glycerol Stock Protocol= 1) Add .5 mL 50% glycerol solution in water and .5 mL cell culture 6-18 hours old (cells preferably still in exponentia...")
(→General Glycerol Stock Protocol)
Line 3: Line 3:
=General Glycerol Stock Protocol=
=General Glycerol Stock Protocol=
 +
<br>
1) Add .5 mL 50% glycerol solution in water and .5 mL cell culture 6-18 hours old (cells preferably still in exponential growth phase) to a 1.5 mL Eppendorf tube.
1) Add .5 mL 50% glycerol solution in water and .5 mL cell culture 6-18 hours old (cells preferably still in exponential growth phase) to a 1.5 mL Eppendorf tube.
 +
<br>
2) If possible, flash freeze in dry ice bathed in 70% ethanol.
2) If possible, flash freeze in dry ice bathed in 70% ethanol.
 +
<br>
3) Store at -80°C.
3) Store at -80°C.

Revision as of 15:38, 2 July 2012


General Glycerol Stock Protocol


1) Add .5 mL 50% glycerol solution in water and .5 mL cell culture 6-18 hours old (cells preferably still in exponential growth phase) to a 1.5 mL Eppendorf tube.
2) If possible, flash freeze in dry ice bathed in 70% ethanol.
3) Store at -80°C.

Retrieved from "http://2012.igem.org/Team:WashU/Protocols/GlycerolStock"