Latest revision as of 00:20, 27 September 2012

Weeks:

June			July				August				September				October				November

this week we have worked extensively on a batch of different manipulations including:

biobricking promotors in Psb1c3 which are:
- P Elastase
- Hsp70
- P Car A
- FLk1
- P CMV
this work included standard pcr amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing.
Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+: this included PCR amplification of seperate genes GFP-AID and Tir1 using then apllying the golden gate protocol over night, followed by trsnformtion, sequencing and characterization of those of PCS2+ in tadpoles
Preparation of chemical Competent Cells

@@ Line 11: / Line 11: @@
                       <li> FLk1</li>
                       <li>P CMV</li></ul>
-this work included standard pcr amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing.</ul>
+this work included standard pcr amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing.
-<li>Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+:</li>
+<li>Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+:
-this included PCR amplification of seperate genes GFP-AID and Tir1 using  then apllying the golden gate protocol over night, followed by trsnformtion, sequencing and characterization of those of PCS2+ in tadpoles,</ul>
+this included PCR amplification of seperate genes GFP-AID and Tir1 using  then apllying the golden gate protocol over night, followed by trsnformtion, sequencing and characterization of those of PCS2+ in tadpoles</li>
-<ul>
-<li>Preparation of chemical Competent Cells</li>
+<li>Preparation of chemical Competent Cells</li></ul>
 </ul>