Team:Evry/Notebook/w14

From 2012.igem.org

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<li>Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+:
<li>Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+:
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this included PCR amplification of seperate genes GFP-AID and Tir1 using  then apllying the golden gate protocol over night, followed by trsnformtion, sequencing and characterization of those of PCS2+ in tadpoles</li>
this included PCR amplification of seperate genes GFP-AID and Tir1 using  then apllying the golden gate protocol over night, followed by trsnformtion, sequencing and characterization of those of PCS2+ in tadpoles</li>
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<li>Preparation of chemical Competent Cells</li>
<li>Preparation of chemical Competent Cells</li>
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Revision as of 00:16, 27 September 2012

Weeks:

June July August September October November

Week 14: 10th September - 16th September

this week we have worked extensively on a batch of different manipulations including:

  • biobricking promotors in Psb1c3 which are:
    • P Elastase
    • Hsp70
    • P Car A
    • FLk1
    • P CMV
    this work included standard pcr amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing.
  • Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+: this included PCR amplification of seperate genes GFP-AID and Tir1 using then apllying the golden gate protocol over night, followed by trsnformtion, sequencing and characterization of those of PCS2+ in tadpoles
  • Preparation of chemical Competent Cells

    • Retrieved from "http://2012.igem.org/Team:Evry/Notebook/w14"