Team:ULB-Brussels/Modeling

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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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<li><a href="https://2012.igem.org/Team:ULB-Brussels"></a></li>
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<li><a href="https://2012.igem.org/Team:ULB-Brussels/Team">&nbsp; Our Team &nbsp;</a></li>
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<li><a href="https://2012.igem.org/Team:ULB-Brussels/Project">&nbsp; The Project &nbsp;</a></li>
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<li><a href="https://2012.igem.org/Team:ULB-Brussels/Parts">&nbsp; Parts submitted &nbsp;</a></li>
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<li><a href="https://2012.igem.org/Team:ULB-Brussels/Safety">&nbsp; Safety &nbsp;</a></li>
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<li><a href="https://2012.igem.org/Team:ULB-Brussels/Attributions">&nbsp; Attributions &nbsp;</a></li>
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<font color=#1D702D;background-color:#FC7F3C;><font Size="100"><p>  Team ULB-Brussels, welcome to our wiki! </p></font>
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!align="center"|[[Team:ULB-Brussels|Home]]
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<h2>Abstract of our project</h2>
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!align="center"|[[Team:ULB-Brussels/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=ULB-Brussels Official Team Profile]
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!align="center"|[[Team:ULB-Brussels/Project|Project]]
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!align="center"|[[Team:ULB-Brussels/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:ULB-Brussels/Modeling|Modeling]]
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!align="center"|[[Team:ULB-Brussels/Notebook|Notebook]]
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!align="center"|[[Team:ULB-Brussels/Attributions|Attributions]]
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If you choose to include a '''Modeling''' page, please write about your modeling adventures here.  This is not necessary but it may be a nice list to include.
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<p>In synthetic biology, one of the main issues scientists and engineers must tackle is biochemical pathways optimization.
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In fact, it is often difficult to predict in which positions the genes of the pathway must be assembled in order to efficiently
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produce the desired molecule.
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<br></br>
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In this project, we are going to develop an exceptional natural tool that could be used to optimize bio-production pathways: 
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the integron. Integrons are genetic platforms which contain (re)movable gene cassettes. These integrons are mostly known to carry
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resistances to antibiotics. They are flanked with recombination sites which allow gene shuffling inside the integron thanks to a  
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specific enzyme: the integrase.
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<br></br>
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As a proof of concept, we are going to produce two antibiotics: Microcin C7 and Microcin B17. The first one inhibits a tRNA synthetase,
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the second a gyrase. These two antibiotic operons encompass respectively 6 and 7 genes.
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<br></br>
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Two bacteria possessing the integron containing the antibiotics production gene cassettes, the integrase and a low resistance to the
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opposite antibiotic will be put in competition. With the integrase, we could change the natural order of the genes in order to optimize
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production. In parallel, this competition experiment will be modeled.</p>
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Revision as of 13:55, 18 September 2012

2012 iGEM - ULB

 

Team ULB-Brussels, welcome to our wiki!

Abstract of our project

In synthetic biology, one of the main issues scientists and engineers must tackle is biochemical pathways optimization. In fact, it is often difficult to predict in which positions the genes of the pathway must be assembled in order to efficiently produce the desired molecule.

In this project, we are going to develop an exceptional natural tool that could be used to optimize bio-production pathways: the integron. Integrons are genetic platforms which contain (re)movable gene cassettes. These integrons are mostly known to carry resistances to antibiotics. They are flanked with recombination sites which allow gene shuffling inside the integron thanks to a specific enzyme: the integrase.

As a proof of concept, we are going to produce two antibiotics: Microcin C7 and Microcin B17. The first one inhibits a tRNA synthetase, the second a gyrase. These two antibiotic operons encompass respectively 6 and 7 genes.

Two bacteria possessing the integron containing the antibiotics production gene cassettes, the integrase and a low resistance to the opposite antibiotic will be put in competition. With the integrase, we could change the natural order of the genes in order to optimize production. In parallel, this competition experiment will be modeled.

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