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Team:Exeter/lab book/3gip/wk6
From 2012.igem.org
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<p><b><u>Monday 13th August</u></b></p> | <p><b><u>Monday 13th August</u></b></p> | ||
| - | <p>See <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6" style="color:#57B947"><u>Operon Construction 13th - 17th August</u></a> of RBS-<i>wbbC</i>(d) (BBa_B0034 + BBa_K764005)</p> | + | <p>See <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6" style="color:#57B947" target="_blank"><u>Operon Construction 13th - 17th August</u></a> of RBS-<i>wbbC</i>(d) (BBa_B0034 + BBa_K764005)</p> |
<p></p><br> | <p></p><br> | ||
<p><b><u>Tuesday 14th August</u></b></p> | <p><b><u>Tuesday 14th August</u></b></p> | ||
| - | <p>See <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6" style="color:#57B947"><u>Operon Construction 13th - 17th August</u></a> of RBS-<i>wbbC</i>(d) (BBa_B0034 + BBa_K764005)</p> | + | <p>See <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6" style="color:#57B947" target="_blank"><u>Operon Construction 13th - 17th August</u></a> of RBS-<i>wbbC</i>(d) (BBa_B0034 + BBa_K764005)</p> |
<p></p><br> | <p></p><br> | ||
Revision as of 22:03, 26 September 2012
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The 3-Gene Inducible Plasmid: 13th - 17th August 2012 Monday 13th August See Operon Construction 13th - 17th August of RBS-wbbC(d) (BBa_B0034 + BBa_K764005) Tuesday 14th August See Operon Construction 13th - 17th August of RBS-wbbC(d) (BBa_B0034 + BBa_K764005) Wednesday 15th August Morning BBa_J13002 + wbnK BBa_B0014 in pSB1T3 wfcA + BBa_B0014 in pSB1T3 wbnJ + BBa_B0014 in pSB1T3 BBa_B0034 wbbC + BBa_B0014 in pSB1T3 Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a good pellet formed. After the neutralisation solution had been added, the eppendorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation. 40ul of water was added instead of 50ul in order to make sure the concentration of DNA increased. All were nanodropped and only wfcA + BBa_B0014 and wbnJ + BBa_B0014 gave good concentrations. Wednesday 15th August – Friday 17th August Preparation for presentation so no lab work. |
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