Team:KAIST Korea/Plus Attribution

From 2012.igem.org

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<span id='sub-title'>Data for Our Favorite New Parts</span></br></br>
<span id='sub-title'>Data for Our Favorite New Parts</span></br></br>
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<li style="margin:0px 0px 0px 20px"><a target="_blank" href='http://partsregistry.org/Part:BBa_K907000'>Main Page</a> - <i>Bacteriophage Bxb1 gp35, DNA integrase(Bxb1_Int), BBa_K907000</i>: </br>A serine integrase family protein which recognizes specific DNA sequences and recombines dsDNA.</li></br>
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<li style="margin:0px 0px 0px 20px"><i>Prof. Byung-Kwan Cho,</i>: </br> PI of Systems and Synthetic Biology Lab., has supervised the whole project we have done so far. He has advised about our direction and purpose of research, helped us not to drift.</li></br>
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<li style="margin:0px 0px 0px 20px"><a target="_blank" href='http://partsregistry.org/Part:BBa_K907002'>Main Page</a> - <i>Binary Signal Generator, RBS(reverse) - attB - Promoter - attP – RBS, BBa_K907002</i>: </br>Promotes transcription and translation of two different proteins one at a time when work with BBa_K907000 and BBa_K907001 to generate binary signal.</li></br>
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<li style="margin:0px 0px 0px 20px"><i>Yoseb Song,</i>: </br>graduate student of Systems and Synthetic Biology Lab., advised about detailed trouble-shootings and experimental methods.</li></br>
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<li style="margin:0px 0px 0px 20px"><a target="_blank" href='http://partsregistry.org/Part:BBa_K907004'>Main Page</a> - <i>Dual Phase Protein Generator (GFP default), BBa_K907004</i>: </br>Derivative of BBa_K907002 mRFP and GFP are attached to both ends of BBa_K907002 to generate binary signal via fluorescent proteins.</li></br>
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<li style="margin:0px 0px 0px 20px"><i>Juhee Kim,</i>: </br>graduate student of Systems and Synthetic Biology Lab., helped us with making video clip representing our project by drawing lovely figures.</li></br>
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The engineered bFMO(Bacterial Flavin-containing monooxygenase) gene was kindly donated by <i>Prof. Duhee Bang</i> in Yonsei University, Republic of Korea.
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Biochem Biophys Res Commun. 2003 Jul 11;306(4):930-6.
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<li style="margin:0px 0px 0px 20px">[PubMed] <a target="_blank" href='http://www.ncbi.nlm.nih.gov/pubmed/12821131?dopt=Abstract&holding=npg'>http://www.ncbi.nlm.nih.gov/pubmed/12821131?dopt=Abstract&holding=npg</a>
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<li style="margin:0px 0px 0px 20px"><i>Hyewon Jeong,</i>: </br>our team leader, is the one who recruited our team members. She has arranged all our team meetings, seminars, and some hangouts, too. She came up with brillant ideas about the video clip. She also participated a lot in Gibson assembly.</li></br>
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<li style="margin:0px 0px 0px 20px"><i>Donghui Choe & Sooin Lee</i>: </br>have designed the parts involved in Flip & Flop. They also wrote documents for these parts, drew every related figure. Donghui, specialist in designing figures, designed the logo for Flip & Flop.</li></br>
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<li style="margin:0px 0px 0px 20px"><i>Jihwan Chun & Namil Lee</i>: </br>have done most of the cell culture works for the research. They successfully completed TOPO cloning and examined the expression of cloned genes. They also arranged human practice events and gave out lectures to middle school and high school students.</li></br>
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<li style="margin:0px 0px 0px 20px"><i>Sulli Lee & Jongho Lee</i>: </br>are specialists in software and programming. They have constructed our team webpage and team wiki. In addition, they are doing the modeling of our module.</li></br>
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Oligonucleotides have been synthesized by Genotech. Gene syntheses were done by Bioneer. DNA Sequencing services were done by Macrogen.
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Revision as of 21:33, 26 September 2012

KAIST Korea 2012 iGEM

Plus : Attribution

Attribution Page

All of our experiments are done in Systems and Synthetic Biology Laboratory in KAIST under the guidance of Prof. Byung-Kwan Cho.

Data for Our Favorite New Parts

  1. Prof. Byung-Kwan Cho,:
    PI of Systems and Synthetic Biology Lab., has supervised the whole project we have done so far. He has advised about our direction and purpose of research, helped us not to drift.

  2. Yoseb Song,:
    graduate student of Systems and Synthetic Biology Lab., advised about detailed trouble-shootings and experimental methods.

  3. Juhee Kim,:
    graduate student of Systems and Synthetic Biology Lab., helped us with making video clip representing our project by drawing lovely figures.

    4. The engineered bFMO(Bacterial Flavin-containing monooxygenase) gene was kindly donated by Prof. Duhee Bang in Yonsei University, Republic of Korea. Biochem Biophys Res Commun. 2003 Jul 11;306(4):930-6.
  4. [PubMed] http://www.ncbi.nlm.nih.gov/pubmed/12821131?dopt=Abstract&holding=npg
  5. Hyewon Jeong,:
    our team leader, is the one who recruited our team members. She has arranged all our team meetings, seminars, and some hangouts, too. She came up with brillant ideas about the video clip. She also participated a lot in Gibson assembly.

  6. Donghui Choe & Sooin Lee:
    have designed the parts involved in Flip & Flop. They also wrote documents for these parts, drew every related figure. Donghui, specialist in designing figures, designed the logo for Flip & Flop.

  7. Jihwan Chun & Namil Lee:
    have done most of the cell culture works for the research. They successfully completed TOPO cloning and examined the expression of cloned genes. They also arranged human practice events and gave out lectures to middle school and high school students.

  8. Sulli Lee & Jongho Lee:
    are specialists in software and programming. They have constructed our team webpage and team wiki. In addition, they are doing the modeling of our module.

    9. Oligonucleotides have been synthesized by Genotech. Gene syntheses were done by Bioneer. DNA Sequencing services were done by Macrogen.

Data for Pre-existing Parts

  1. Experience – Highly engineered mutant of red fluorescent protein from Discosoma striata (coral), BBa_E1010 (Endy Lab, 2004):
    BBa_E1010 was successfully used to produce mRFP in E.coli strain MG1655 in LB or M9 minimal media under the control of BBa_K907005.

  2. Experience – Green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Endy Lab, 2004):
    BBa_E0040 was successfully used to produce GFP in E.coli strain MG1655 in LB or M9 minimal media under the control of BBa_K907004.
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