"
Team:Evry-Genopole/Notebook/June/29
From 2012.igem.org
(Difference between revisions)
Chr.karine (Talk | contribs) |
Chr.karine (Talk | contribs) |
||
| Line 11: | Line 11: | ||
Digested RFP, YFP, CFP, GFP, pCS2+ (from 28 june) : 10microL sample + 2microL loading buffer<br/> | Digested RFP, YFP, CFP, GFP, pCS2+ (from 28 june) : 10microL sample + 2microL loading buffer<br/> | ||
'''=> 100V, 1h'''<br/> | '''=> 100V, 1h'''<br/> | ||
| - | '''Result:'''no DNA detectable, '''Solution:''' to redo the step of digestion with more DNA<br/> | + | '''Result:'''no DNA detectable, '''Solution:''' to redo the step of digestion with more DNA<br/><br/> |
<FONT SIZE=3>'''DNA digestion'''</FONT> under construction<br/> | <FONT SIZE=3>'''DNA digestion'''</FONT> under construction<br/> | ||
4ul of CFP, GFP, RFP, YFP dosed on 28 june <br/> | 4ul of CFP, GFP, RFP, YFP dosed on 28 june <br/> | ||
| - | 3ul of pSC2+ dosed on 28 june <br/> | + | 3ul of pSC2+ dosed on 28 june <br/><br/> |
<FONT SIZE=3>'''Gel migration'''</FONT> <br/> | <FONT SIZE=3>'''Gel migration'''</FONT> <br/> | ||
[[File:Photo_GelMigration2906.jpeg|left|200px]] | [[File:Photo_GelMigration2906.jpeg|left|200px]] | ||
| Line 23: | Line 23: | ||
Digested RFP, YFP, CFP, GFP, pCS2+ (from 29 june) : 15ul sample + 3ul loading buffer<br/> | Digested RFP, YFP, CFP, GFP, pCS2+ (from 29 june) : 15ul sample + 3ul loading buffer<br/> | ||
'''=> 100V, 1h'''<br/> | '''=> 100V, 1h'''<br/> | ||
| - | '''Result:''' no RFP detectable<br/> | + | '''Result:''' no RFP detectable<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/> |
| + | <FONT SIZE=3>'''Gel extraction'''</FONT> under construction<br/> | ||
| + | <br/> | ||
| + | <FONT SIZE=3>'''Ligation'''</FONT> under construction<br/> | ||
| + | <br/> | ||
Revision as of 10:14, 30 June 2012
Promoters & Reporters workgroup
Gel preparation
25mL agarose+TAE (frigo) + 2microL BET
Sample preparation
DNA Ladder 1kB: 1microL ladder + 1microL loading buffer + 4microL ddH2O
Digested RFP, YFP, CFP, GFP, pCS2+ (from 28 june) : 10microL sample + 2microL loading buffer
=> 100V, 1h
Result:no DNA detectable, Solution: to redo the step of digestion with more DNA
DNA digestion under construction
4ul of CFP, GFP, RFP, YFP dosed on 28 june
3ul of pSC2+ dosed on 28 june
Gel migration
Gel preparation
50mL agarose+TAE (frigo) + 4ul BET
Sample preparation
DNA Ladder 1kB: 1ul ladder + 1ul loading buffer + 4ul ddH2O
Digested RFP, YFP, CFP, GFP, pCS2+ (from 29 june) : 15ul sample + 3ul loading buffer
=> 100V, 1h
Result: no RFP detectable
Gel extraction under construction
Ligation under construction
