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H₂ E.coli: Discussion

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The Efffects of the E363G Mutation in fhlA Gene on Hydrogen Production

Sanchez-Torres et al. obtained by random mutagenesis several mutants of the transcriptional factor gene fhlA in which hydrogen gas production is enhanced.[1] Among these mutants, the E363G mutation allowed E.coli cells to produce hydrogen six times more than the original fhlA under anaerobic conditions with 20 mM formate. Therefore, we made a BioBrick? named fhlA-E363G by introducing the same mutation into the original wild-type fhlA part which we BioBricked. Our aim is to make E. coli cells which are capable of producing hydrogen in a glucose-rich environment. The over-expression of wild-type fhlA indeed promoted hydrogen production enormously compared to cells transformed with an empty plasmid. In an attempt to further increase hydrogen production, we compared the hydrogen production of two E. coli strains which over-expresses wild-type fhlA and fhlA-E363G in a glucose-rich environment. As a result, the fhlA-E363G expressing strain generated less hydrogen gas than the wild-type fhlA expressing strain. It seems that, under glucose-rich conditions, the E363G mutation in fhlA does not lead to an increase of hydrogen production compared to wild-type fhlA. [1]Viviana Sanchez-Torres, Toshinari Maeda, and Thomas K. Wood. Protein Engineering of the Transcriptional Activator FhlA To Enhance Hydrogen Production in Escherichia coli. APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75, 5639–5646. 2009.

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 == The Efffects of the E363G Mutation in fhlA Gene on Hydrogen Production ==
-Sanchez-Torres et al. obtained by random mutagenesis several mutants of the transcriptional factor gene fhlA in which hydrogen gas production is enhanced.[1] Among these mutants, the E363G mutation allowed ''E.coli'' cells to produce hydrogen six times more than the original fhlA under anaerobic conditions with 20 mM formate. Therefore, we made a BioBrick? named fhlA-E363G by introducing the same mutation into the original wild-type fhlA part which we BioBricked?. Our aim is to make ''E. coli'' cells which are capable of producing hydrogen in a glucose-rich environment. The over-expression of wild-type fhlA indeed promoted hydrogen production enormously compared to cells transformed with an empty plasmid. In an attempt to further increase hydrogen production, we compared the hydrogen production of two ''E. coli'' strains which over-expresses wild-type fhlA and fhlA-E363G in a glucose-rich environment. As a result, the fhlA-E363G expressing strain generated less hydrogen gas than the wild-type fhlA expressing strain. It seems that, under glucose-rich conditions, the E363G mutation in fhlA does not lead to an increase of hydrogen production compared to wild-type fhlA.
+Sanchez-Torres et al. obtained by random mutagenesis several mutants of the transcriptional factor gene fhlA in which hydrogen gas production is enhanced.[1] Among these mutants, the E363G mutation allowed ''E.coli'' cells to produce hydrogen six times more than the original fhlA under anaerobic conditions with 20 mM formate. Therefore, we made a BioBrick? named fhlA-E363G by introducing the same mutation into the original wild-type fhlA part which we BioBricked.
+Our aim is to make ''E. coli'' cells which are capable of producing hydrogen in a glucose-rich environment. The over-expression of wild-type fhlA indeed promoted hydrogen production enormously compared to cells transformed with an empty plasmid. In an attempt to further increase hydrogen production, we compared the hydrogen production of two ''E. coli'' strains which over-expresses wild-type fhlA and fhlA-E363G in a glucose-rich environment. As a result, the fhlA-E363G expressing strain generated less hydrogen gas than the wild-type fhlA expressing strain. It seems that, under glucose-rich conditions, the E363G mutation in fhlA does not lead to an increase of hydrogen production compared to wild-type fhlA.
 [1]Viviana Sanchez-Torres, Toshinari Maeda, and Thomas K. Wood. Protein Engineering of the Transcriptional Activator FhlA To Enhance Hydrogen Production in Escherichia coli. APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75, 5639–5646. 2009.

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