Team:SJTU-BioX-Shanghai/Project/project2.1
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[[Image:12SJTU_violacein_production_ratio.JPG|thumb|320px|left|''Fig.2'' :The ratio of violacein with deoxyviolacein from membrane complex system with or without light induction]] | [[Image:12SJTU_violacein_production_ratio.JPG|thumb|320px|left|''Fig.2'' :The ratio of violacein with deoxyviolacein from membrane complex system with or without light induction]] | ||
[[Image:12SJTU_Violacein_production3_Ratio.JPG|thumb|350px|right|''Fig.3'' :The ratio of side products with desired products from membrane complex system and control system]] | [[Image:12SJTU_Violacein_production3_Ratio.JPG|thumb|350px|right|''Fig.3'' :The ratio of side products with desired products from membrane complex system and control system]] | ||
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These results fully confirm the idea of membrane protein assembly and the great role it plays in the switch of reaction. In addition to the anticipated realization of reaction switch, we were satisfied to observe significant suppression of the side products (deoxychromoviridans), which was primarily due to fact that PVA was instantly transferred to deoxyviolacein or violacein by adjacent Vio D and Vio C. | These results fully confirm the idea of membrane protein assembly and the great role it plays in the switch of reaction. In addition to the anticipated realization of reaction switch, we were satisfied to observe significant suppression of the side products (deoxychromoviridans), which was primarily due to fact that PVA was instantly transferred to deoxyviolacein or violacein by adjacent Vio D and Vio C. | ||
[[Image:12SJTU_violacein_products2.jpg|thumb|400px|center|''Fig.4'' : HPLC results of the bacterial extraction containing violacein biosynthetic pathway products. HPLC results indicate that violacein is prodominant in the bacteria extraction with the light induction. Without light induction, deoxyviolacein is dominant in the sample. The amount of deoxychromoviridans is greatly decreased in the bacteria with membrane system. ]] | [[Image:12SJTU_violacein_products2.jpg|thumb|400px|center|''Fig.4'' : HPLC results of the bacterial extraction containing violacein biosynthetic pathway products. HPLC results indicate that violacein is prodominant in the bacteria extraction with the light induction. Without light induction, deoxyviolacein is dominant in the sample. The amount of deoxychromoviridans is greatly decreased in the bacteria with membrane system. ]] | ||
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Although the theoretical production of violacein biosynthetic pathway composed of only three pigments, the separation result of HPLC depends on many different factors, including the purity of extraction, stationery phase type, the detector wavelength, etc. However, we did not have time to perform any optimizations with respect to extraction protocol, HPLC conditions etc., therefore the final result of HPLC could be much more legible with less interference of noise peak. Despite that the experiment was repeated more than three times with similar results. | Although the theoretical production of violacein biosynthetic pathway composed of only three pigments, the separation result of HPLC depends on many different factors, including the purity of extraction, stationery phase type, the detector wavelength, etc. However, we did not have time to perform any optimizations with respect to extraction protocol, HPLC conditions etc., therefore the final result of HPLC could be much more legible with less interference of noise peak. Despite that the experiment was repeated more than three times with similar results. |
Revision as of 18:06, 26 September 2012