Team:KAIT Japan/Notebook

From 2012.igem.org

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:<big>'''Date:9/20'''</big> Restriction enzyme processing
:<big>'''Date:9/20'''</big> Restriction enzyme processing
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:<big>'''Date:9/21'''</big> Ligation
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:<big>'''Date:9/21'''</big> Ligation and PCR,Elecrtophoresis
:<big>'''Date:9/23'''</big> PCR
:<big>'''Date:9/23'''</big> PCR

Revision as of 17:55, 26 September 2012

KAIT Japan2012 logo.png
KAIT Japan2012 Sub.png
Kaitjapan iGEM official.logo.png


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Home

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Project

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Parts

Kaitjapan protocol.png

Protocol

Kaitjapan notebook.png

Notebook

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Results

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Safety

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Human Practice

Kaitjapan team.png

Team

Notebook

Our team pushed forward an experiment while making trial and error every day.

Creating parts of Methylation

Creating parts of Tar

Date:9/13 PCR and Colony PCR
Date:9/14 Electrophoretic confirmation
Date:9/15 Restriction enzyme processing
Date:9/16 Electrophoresis of the restriction enzyme processing product and Restriction enzyme processing
Date:9/17 Refinement of DNA and Ligation,Transformation
Date:9/18 Making of a master plate and Preculture,Recultivation
Date:9/19 Colony PCR and Electrophoresis
Date:9/20 Ligation and Transformation
Date:9/21 Inoculation
Date:9/22 Making of a master plate and Colony PCR,Electrophoresis,Preculture
Date:9/23 Electrophoretic confirmation and Electrophoresis of the PCR
Date:9/24 Miniprep and Electrophoresis
Date:9/25 PCR of the miniprep product and Electrophoresis

Creating parts of EnvZ

Date:9/10 Design of the primer and PCR,Elecrtophoresis,Miniprep
Date:9/13 PCR and Elecrtophoresis
Date:9/18 Design of the primer
Date:9/19 PCR and Elecrtophoresis
Date:9/20 Restriction enzyme processing
Date:9/21 Ligation and PCR,Elecrtophoresis
Date:9/23 PCR
Date:9/24 The refinement of DNA and Restriction enzyme processing,Ligation
Date:9/25 Transformation
Date:9/26 Liquid culture

Creating parts of Azurin

Creating parts of Tar methylation region

Date:8/9 Colony PCR
Date:8/11 The purified DNA
Date:8/13 Confirmed of electrophoresis by PCR product and Ligation of the TA vector
Date:8/14 Transformation
Date:8/25 Miniprep
Date:8/30 Electrophoresis,Refinement of DNA,PCR,Nanodrop
Date:9/1 Miniprep,PCR,Elecrtophoresis
Date:9/2 Meeting
Date:9/5 Miniprep,Nanodrop
Date:9/6 Miniprep,PCR,Electrophoresis
Date:9/10 Colony PCR,Refinement of DNA,c,Miniprep
Date:9/11 Primer design
Date:9/12 Miniprep,Ligation
Date:9/13 Electrophoresis,Refinement of DNA
Date:9/14 Colony PCR
Date:9/15 Ligation
Date:9/16 PCR,Ligation
Date:9/17 PCR,Ligation
Date:9/19 Ligation
Date:9/20 Enzyme inactivation,Ligation
Date:9/22 Transformation,Colony PCR,Electrophoresis,Miniprep
Date:9/23 Colony PCR,Electrophoresis,Streak
Date:9/25 Colony PCR

Creating parts of azurin

Date:8/16 Colony PCR,Ligation
Date:8/18 Colony PCR
Date:8/20 DNA extraction and purification of P.aeruginosa
Date:8/30 Electrophoresis,Refinement of DNA,PCR
Date:9/1 Electrophoresis
Date:9/3 Ligation,Transformation,PCR,Nanodrop
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