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Team:Paris-Saclay/Project/Notebook/Week 7
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Revision as of 20:43, 26 September 2012
Week 7
- PCR in large volume to have a lot a available biobricks
- Treatment with DPN1 to eliminate plasmids
- Gel purification to only keep our interest fragments
- Gibson Assembly method to put together the different biobricks for each construction
- Transformation with assembly products and staggering on a selective medium
- Colony PCR to have results before the transformation results
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