Team:KAIT Japan/Results

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(Difference between revisions)
(→cytoplasmic region of tar)
(→cytoplasmic region of tar)
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:'''Figure1.PCR production from BBa_J22010'''
:'''Figure1.PCR production from BBa_J22010'''
:'''enzyme tag EcoRⅠand PstⅠ　We experimented as following.'''  
:'''enzyme tag EcoRⅠand PstⅠ　We experimented as following.'''  
-
:1.restriction enzyme processing PCR production  
+
:'''1.restriction enzyme processing PCR production'''
-
:2.ligation  
+
:'''2.ligation'''
-
:3.transformation
+
:'''3.transformation'''
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:4.culture on the LB nutrient medium and get colonies.
+
:'''4.culture on the LB nutrient medium and get colonies.'''
-
:5.colony PCR and numbering colonies.
+
:'''5.colony PCR and numbering colonies.'''
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:6.We did miniprep 1,2,4,5.this is colony number
+
:'''6.We did miniprep 1,2,4,5.this is colony number'''
:
:
:[[file:KAIT_Japan-Results-Tar2.jpg]]   
:[[file:KAIT_Japan-Results-Tar2.jpg]]   
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:Figure2.the colony havingytoplasmic region of tar  
+
:'''Figure2.the colony havingytoplasmic region of tar '''
:
:
-
:7.After miniprep we did PCR by using VF2 and VR primer  
+
:'''7.After miniprep we did PCR by using VF2 and VR primer'''
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:8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep :production are tar parts.
+
:'''8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep production are tar parts.'''
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:cytoplasmic region of tar bp is varied. 900bp~1000bp
+
:'''cytoplasmic region of tar bp is varied. 900bp~1000bp'''
:
:
:[[file:KAIT_Japan-Results-Tar3.jpg]]   
:[[file:KAIT_Japan-Results-Tar3.jpg]]   
-
:Figure3 PCR production by using VF and butVR
+
:'''Figure3 PCR production by using VF and butVR'''

Revision as of 16:27, 26 September 2012

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Results

cytoplasmic region of tar

We did PCR so as to get cytoplasmic region of tar.We used primer with restriction.
KAIT Japan-Results-Tar1.jpg
Figure1.PCR production from BBa_J22010
enzyme tag EcoRⅠand PstⅠ　We experimented as following.
1.restriction enzyme processing PCR production
2.ligation
3.transformation
4.culture on the LB nutrient medium and get colonies.
5.colony PCR and numbering colonies.
6.We did miniprep 1,2,4,5.this is colony number
KAIT Japan-Results-Tar2.jpg
Figure2.the colony havingytoplasmic region of tar
7.After miniprep we did PCR by using VF2 and VR primer
8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep production are tar parts.
cytoplasmic region of tar bp is varied. 900bp~1000bp
KAIT Japan-Results-Tar3.jpg
Figure3 PCR production by using VF and butVR
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