Revision as of 16:15, 26 September 2012

Home

Project

Parts

Protocol

Notebook

Results

Safety

Human Practice

Team

Results

We did PCR so as to get cytoplasmic region of tar.We used primer with restriction.

Figure1.PCR production from BBa_J22010

enzyme tag EcoRⅠand PstⅠ　We experimented as following.

1.restriction enzyme processing PCR production

2.ligation

3.transformation

4.culture on the LB nutrient medium and get colonies.

5.colony PCR and numbering colonies.

6.We did miniprep 1,2,4,5.this is colony number

Figure2.the colony havingytoplasmic region of tar

7.After miniprep we did PCR by using VF2 and VR primer

8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep :production are tar parts.

cytoplasmic region of tar bp is varied. 900bp~1000bp

Figure3 PCR production by using VF and butVR

@@ Line 54: / Line 54: @@
 =='''cytoplasmic region of tar'''==
 :We did PCR so as to get cytoplasmic region of tar.We used primer with restriction.
-[[file:KAIT_Japan-Results-Tar1.jpg]]
+:[[file:KAIT_Japan-Results-Tar1.jpg]]
 :Figure1.PCR production from BBa_J22010
 :enzyme tag EcoRⅠand PstⅠ　We experimented as following.
@@ Line 63: / Line 63: @@
 :5.colony PCR and numbering colonies.
 :6.We did miniprep 1,2,4,5.this is colony number
 :
+:[[file:KAIT_Japan-Results-Tar2.jpg]]
 :Figure2.the colony havingytoplasmic region of tar
 :