Team:SDU-Denmark/geneseq

From 2012.igem.org

(Difference between revisions)
Line 265: Line 265:
First primer    Extended    Start codon    Stop codon    Restriction site        Mutagenesis primer
First primer    Extended    Start codon    Stop codon    Restriction site        Mutagenesis primer
<b>1-FFT:</b>
<b>1-FFT:</b>
-
AGGAGGTGTCACCATGCAAACCCCTGAACCCTTTACAGACCTTGAACATGAACCCCACACACCCCTACTGGACCACCACCACAACCCACCACCACAA</br>
+
 
-
ACCACCACAAAACCTTTGTTCACCAGGGTTGTGTCCGGTGTCACCTTTGTTTTATTCTTCTTTGGTTTCGCTATCGTATTCATTGTTCTCAACCAAC</br>
+
-
AGAACTCTTCTGTTCGTATCGTCACCAATTCGGAGAAATCTTTTATAAGGTATTCGCAGACCGATCGCTTGTCGTGGGAACGGACCGCTTTTCATTT</br>
+
-
TCAGCCTGCCAAGAATTTTATTTACGATCCAGATGGTCAGTTGTTTCACATGGGCTGGTACCATATGTTCTATCAATACAACCCATACGCACCGGTT</br>
+
-
TGGGGCAATATGTCATGGGGTCACTCAGTGTCCAAAGACATGATCAACTGGTACGAGCTGCCAGTCGCTATGGTCCCGACCGAATGGTATGATATCG</br>
+
-
AGGGCGTCTTATCCGGGTCTACCACGGTCCTTCCAAACGGTCAGATCTTTGCATTGTATACTGGGAACGCTAATGATTTTTCCCAATTACAATGCAA</br>
+
-
AGCTGTACCCGTAAACTTATCTGACCCGCTTCTTATTGAGTGGGTCAAGTATGAGGATAACCCAATCCTGTACACTCCACCAGGGATTGGGTTAAAG</br>
+
-
GACTATCGGGACCCGTCAACAGTCTGGACAGGTCCCGATGGAAAGCATAGGATGATCATGGGAACTAAACGTGGCAATACAGGCATGGTACTTGTTT</br>
+
-
ACTATACCACTGATTACACGAACTACGAGTTGTTGGATGAGCCGTTGCACTCTGTTCCCAACACCGATATGTGGGAATGCGTCGACTTTTACCCGGT</br>
+
-
TTCGTTAACCAATGATAGTGCACTTGATATGGCGGCCTATGGGTCGGGTATCAAACACGTTATTAAAGAAAGTTGGGAGGGACATGGAATGGATTGG</br>
+
-
TATTCAATCGGGACATATGACGCGATAAATGATAAATGGACTCCCGATAACCCGGAACTAGATGTCGGTATCGGGTTACGGTGCGATTACGGGAGGT</br>
+
-
TTTTTGCATCAAAGAGTCTTTATGACCCATTGAAGAAAAGGAGGATCACTTGGGGTTATGTTGGAGAATCAGATAGTGCTGATCAGGACCTCTCAAG</br>
+
-
AGGATGGGCTACTGTTTATAATGTTGGAAGAACAATTGTACTAGATAGAAAGACCGGGACCCATTTACTTCATTGGCCCGTTGAGGAAGTCGAGAGT</br>
+
-
TTGAGATACAACGGTCAGGAGTTTAAAGAGATCAAGCTAGAGCCCGGTTCAATCATTCCACTCGACATAGGCACGGCTACACAGTTGGACATAGTTG</br>
+
-
CAACATTTGAGGTGGATCAAGCAGCGTTGAACGCGACAAGTGAAACCGATGATATTTATGGTTGCACCACTAGCTTAGGTGCAGCCCAAAGGGGAAG</br>
+
-
TTTGGGACCATTTGGTCTTGCGGTTCTAGCCGATGGAACCCTTTCTGAGTTAACTCCGGTTTATTTCTATATAGCTAAAAAGGCAGATGGAGGTGTG</br>
+
-
TCGACACATTTTTGTACCGATAAGCTAAGGTCATCACTAGATTATGATGGGGAGAGAGTGGTGTATGGGGGCACTGTTCCTGTGTTAGATGATGAAG</br>
+
-
AACTCACAATGAGGCTATTGGTGGATCATTCGATAGTGGAGGGGTTTGCGCAAGGAGGAAGGACGGTTATAACATCAAGGGCGTATCCAACAAAAGC</br>
+
-
GATATACGAACAAGCGAAGCTGTTCTTGTTCAACAACGCCACAGGTACGAGTGTGAAAGCATCTCTCAAGATTTGGCAAATGGCTTCTGCACCAATT</br>
+
-
CATCAATACCCTTTTTAATTACCGGCTATCGCTATCCTTTTTGTTATTG</br>
+

Revision as of 14:52, 26 September 2012

iGEM TEAM ::: SDU-DENMARK courtesy of NIAID



Standard restriction sites:


EcoRI:          5' GAATTC
                      3' CTTAAG

XbaI:            5' T*CTAGA
                      3' AGATC*T

SpeI:             5' A*CTAGT
                      3' TGATC*A

PstI:              5' CTGCAG
                      3' GACGTC

Our plant genes:

First primer Extended Start codon Stop codon Restriction site Mutagenesis primer 1-FFT:

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